sampleSizeBudgetCalculation.restrictedDoublets: Estimate possible sample size depending on the total cost and...

In heiniglab/scPower: Experimental design framework for scRNAseq population studies (eQTL and DE)

Estimate possible sample size depending on the total cost and the other parameters (with a restricted number of cells per lane)

Description

Variant of sampleSizeBudgetCalculation, which restricts the cells per lane instead the individuals per lane.

Usage

sampleSizeBudgetCalculation.restrictedDoublets(
  nCells,
  readDepth,
  totalCost,
  costKit,
  cellsPerLane,
  costFlowCell,
  readsPerFlowcell,
  reactionsPerKit = 6
)

Arguments

nCells	Cells per individual
readDepth	Read depth per cell
totalCost	Experimental budget
costKit	Cost for one 10X kit
cellsPerLane	Number of cells sequenced per lane
costFlowCell	Cost of one flow cells for sequencing
readsPerFlowcell	Number reads that can be sequenced with one flow cell
reactionsPerKit	Reactions (=lanes) per kit, defines the total number of tested individuals per kit

Value

Number of samples that can be measured with this budget and other parameters

heiniglab/scPower documentation built on Jan. 9, 2025, 12:13 p.m.