RNAseqDegs_limma | R Documentation |
Helper Function For DEG Analysis of RNA-seq Data using limma + Voom
RNAseqDegs_limma(counts, phenodata, complist, lfc, qval = 0.05)
counts |
Processed gene expression count data |
phenodata |
pData that provides batch & experimental conditions |
complist |
a vector of multiple comparisons in the format of contrasts in limma (e.g. c("A-B", "A-C", "B-C")) |
lfc |
(optional) log fold change constraints for DEGs |
qval |
q-value cutoff for DEG analysis (default: 0.05) |
DEresult: a list of DEG results, including those for each single comparison and those for the overall comparison. Each DEG result is in the format of A list containing: table: table of DEG results. rank_vector: a vector of t-statistics for every gene. degs: a vector of gene symbols for DEGs. e: expression data (CPM). e_batch: batch corrected expression.
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