RNAseqDegs_limma: Helper Function For DEG Analysis of RNA-seq Data using limma...

View source: R/DEG_Analysis.R

RNAseqDegs_limmaR Documentation

Helper Function For DEG Analysis of RNA-seq Data using limma + Voom

Description

Helper Function For DEG Analysis of RNA-seq Data using limma + Voom

Usage

RNAseqDegs_limma(counts, phenodata, complist, lfc, qval = 0.05)

Arguments

counts

Processed gene expression count data

phenodata

pData that provides batch & experimental conditions

complist

a vector of multiple comparisons in the format of contrasts in limma (e.g. c("A-B", "A-C", "B-C"))

lfc

(optional) log fold change constraints for DEGs

qval

q-value cutoff for DEG analysis (default: 0.05)

Value

DEresult: a list of DEG results, including those for each single comparison and those for the overall comparison. Each DEG result is in the format of A list containing: table: table of DEG results. rank_vector: a vector of t-statistics for every gene. degs: a vector of gene symbols for DEGs. e: expression data (CPM). e_batch: batch corrected expression.


lusystemsbio/NetAct documentation built on Sept. 7, 2024, 8:34 p.m.