cellCycleFractIntegrDNAInt: Derive cell cycle fractions from nuclear DNA intensity...

In mknoll/cmoRe: Analysis of CellProfiler acquired data for disease profiling and drug discovery.

Derive cell cycle fractions from nuclear DNA intensity measurements

Description

Calculates cell-cycle fractions (G1, G2) of cells based on a nuclear DNA intensity variable; defaults to "Intensity_IntegratedIntensity_DNA".

Usage

cellCycleFractIntegrDNAInt(
  data,
  var = "Intensity_IntegratedIntensity_DNA.nucl",
  nBoot = 100,
  lowerCL = 0.1,
  upperCL = 0.9,
  log = F,
  cutUpper = NULL,
  xMinGlobMax = 0,
  xMaxGlobMax = NULL,
  no_cores = NULL,
  ..
)

Arguments

data	data.frame containing at least the columns TREATMENT, VERSUCH, PLATTE and the variable used for DNA cell cycle inference (see parameter var)
var	Variable in which the nuclear integrated dna intensity is stored. Default is "Intensity_IntegratedIntensity_DNA.nucl"
nBoot	number of runs to determine minima/maxima to derive cutoffs
lowerCL	lower confidence level, default: 0.1
upperCL	upper confidence level, default: 0.9
cutUpper	use only data below a certain cutoff, default: NULL -> not used

Value

data.frame containing the computed values

mknoll/cmoRe documentation built on Nov. 18, 2022, 4:01 p.m.