get_numeric_vertex_attributes <- function(sc.data, sel.graph)
{
G <- sc.data$graphs[[sel.graph]]
d <- get.data.frame(G, what = "vertices")
num <- sapply(d, function(x) {is.numeric(x) && !any(is.na(x))})
v <- list.vertex.attributes(G)[num]
exclude <- c("x", "y", "cellType", "type", "groups", "popsize", "r", "g", "b", "size", "DNA1", "DNA2", "BC1", "BC2", "BC3", "BC4", "BC5", "BC6", "Time", "Cell_length", "Cisplatin", "beadDist", "highest_scoring_edge")
return(v[!(v %in% exclude)])
}
plot_cluster <- function(data, cluster, graph.name, col.names)
{
G <- data$graphs[[graph.name]]
gated_data <- data$landmarks.data
clustered_data <- data$clustered.data[[graph.name]]
names(clustered_data) <- gsub("^X", "", names(clustered_data))
names(gated_data) <- gsub("^X", "", names(gated_data))
clustered_data <- clustered_data[, c(col.names, "cellType")]
gated_data <- gated_data[, c(col.names, "cellType")]
land <- V(G)[nei(V(G)$Label == cluster)]$Label
temp <- gated_data[gated_data$cellType %in% land,]
clus.num <- as.numeric(gsub("c", "", cluster))
temp <- rbind(temp, clustered_data[clustered_data$cellType == clus.num,])
temp <- melt(temp, id.vars = "cellType")
temp$variable <- as.factor(temp$variable)
p <- ggplot(aes(x = value, color = cellType), data = temp) + geom_density() + facet_wrap(~variable, scales = "free")
return(p)
}
my_load <- function(f_name)
{
con <- file(f_name, "rb")
retval <- unserialize(con)
close(con)
return(retval)
}
rescale_size <- function(max.size, min.size, max.val, x)
{
return(((max.size - min.size) * x) / max.val + min.size);
}
get_vertex_size <- function(sc.data, sel.graph, figure.width)
{
G <- sc.data$graphs[[sel.graph]]
ret <- V(G)$popsize / sum(V(G)$popsize, na.rm = T)
ret <- rescale_size(60, 2, sc.data$dataset.statistics$max.marker.vals[["popsize.relative"]], ret)
ret[V(G)$type == 1] <- 8
return(ret)
}
get_graph_centering_transform <- function(x, y, svg.width, svg.height)
{
padding <- 50
G.width <- max(x) - min(x)
G.height <- max(y) - min(y)
scaling <- max(c(G.width / (svg.width - (padding * 2)), G.height / (svg.height - (padding * 2))))
x <- x / scaling
y <- y / scaling
offset.y <- min(y) - padding
graph.x.center <- (min(x) + max(x)) / 2
offset.x <- graph.x.center - (svg.width / 2)
return(list(offset.x = offset.x, offset.y = offset.y, scaling = scaling))
}
get_graph <- function(sc.data, sel.graph, trans_to_apply)
{
G <- sc.data$graphs[[sel.graph]]
edges <- data.frame(get.edgelist(G, names = F) - 1)
colnames(edges) <- c("source", "target")
svg.width <- 1200
svg.height <- 800
svg.center <- c(svg.width / 2, svg.height / 2)
x <- V(G)$x
y <- V(G)$y
y <- -1 * y
x <- x + abs(min(x))
y <- y + abs(min(y))
num.landmarks <- sum(V(G)$type == 1)
trans <- get_graph_centering_transform(x[V(G)$type == 1], y[V(G)$type == 1], svg.width, svg.height)
x <- (x / trans$scaling) - trans$offset.x
y <- (y / trans$scaling) - trans$offset.y
vertex.size <- get_vertex_size(sc.data, sel.graph, svg.width)
edges <- cbind(edges, x1 = x[edges[, "source"] + 1], x2 = x[edges[, "target"] + 1])
edges <- cbind(edges, y1 = y[edges[, "source"] + 1], y2 = y[edges[, "target"] + 1])
edges <- cbind(edges, id = 1:nrow(edges))
#print(G)
ret <- list(names = V(G)$Label, size = vertex.size / trans$scaling, type = V(G)$type, highest_scoring_edge = V(G)$highest_scoring_edge, X = x, Y = y, trans_to_apply = trans_to_apply)
ret <- c(ret, edges = list(edges))
return(ret)
}
get_color_for_marker <- function(sc.data, sel.marker, sel.graph, color.scaling)
{
G <- sc.data$graphs[[sel.graph]]
norm.factor <- NULL
v <- get.vertex.attribute(G, sel.marker)
if(color.scaling == "global")
norm.factor <- sc.data$dataset.statistics$max.marker.vals[[sel.marker]]
else if(color.scaling == "local")
norm.factor <- max(v)
a = "#E7E7E7"
b = "#E71601"
f <- colorRamp(c(a, b), interpolate = "linear")
v <- f(v / norm.factor) #colorRamp needs an argument in the range [0, 1]
v <- apply(v, 1, function(x) {sprintf("rgb(%s)", paste(round(x), collapse = ","))})
return(v)
}
get_pubmed_references <- function(sc.data, sel.graph, node.label)
{
G <- sc.data$graphs[[sel.graph]]
ret <- ""
if("desc" %in% list.vertex.attributes(G))
{
ret <- sprintf("List of references for landmark %s:<br>", gsub(".fcs", "", node.label))
v <- strsplit(V(G)[V(G)$Label == node.label]$desc, ",")[[1]]
v <- paste(sapply(v, function(x) {sprintf("PMID: <a href='http://www.ncbi.nlm.nih.gov/pubmed/%s' target='_blank'>%s</a><br>", x, x) }), collapse = "")
ret <- paste(ret, v, sep = "")
}
return(HTML(ret))
}
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