R/processReads.R

#' Process reads from High-Troughtput Sequencing experiments
#'
#' This method allows the processment of NGS nucleosome reads from different
#' sources and a basic manipulation of them. The tasks includes the correction
#' of strand-specific single-end reads and the trimming of reads to a given
#' length.
#'
#' This function reads a [ShortRead::AlignedRead] or a [GenomicRanges::GRanges]
#' object containing the position, length and strand of the sequence reads.
#'
#' It allows the processment of both paired and single ended reads. In the case
#' of single end reads this function corrects the strand-specific mapping by
#' shifting plus strand reads and minus strand reads towards a middle position
#' where both strands are overlaped. This is done by accounting the expected
#' fragment length (`fragmentLen`).
#'
#' For paired end reads, mononucleosomal reads could extend more than expected
#' length due to mapping issues or experimental conditions. In this case, the
#' `fragmentLen` variable sets the threshold from which reads longer than it
#' should be ignored.
#'
#' If no value is supplied for `fragmentLen` it will be calculated
#' automatically (increasing the computing time) using `fragmentLenDetect` with
#' default parameters. Performance can be increased by tunning
#' `fragmentLenDetect` parameteres in a separated call and passing its result
#' as `fragmentLen` parameter.
#'
#' In some cases, could be useful trim the reads to a shorter length to improve
#' the detection of nucleosome dyads, easing its detection and automatic
#' positioning. The parameter `trim` allows the selection of how many
#' nucleotides select from each read.
#'
#' A special case for single-ended data is setting the `trim` to the same
#' value as `fragmentLen`, so the reads will be extended strand-wise towards
#' the 3' direction, creating an artificial map comparable with
#' paired-ended data. The same but opposite can be performed with paired-end
#' data, setting a `trim` value equal to the read length from paired ended, so
#' paired-ended data will look like single-ended.
#'
#' @param data Sequence reads objects, probably imported using other packages
#'   as `ShortRead`. Allowed object types are [ShortRead::AlignedRead] and
#'   [GenomicRanges::GRanges] with a `strand` attribute.
#' @param type Describes the type of reads. Values allowed are `single` for
#'   single-ended reads and `paired` for paired-ended.
#' @param fragmentLen Expected original length of the sequenced fragments. See
#'   details.
#' @param trim Length to trim the reads (or extend them if `trim` > read
#'   length)
#' @param \dots Other parameters passed to `fragmentLenDetect` if no fixed
#'   `fragmentLen` is given.
#'
#' @return [GenomicRanges::GRanges] containing the aligned/trimmed individual
#'   reads.
#'
#' @note **IMPORTANT**: this information is only used to correct possible
#' strand-specific mapping, this package doesn't link the two ends of paired
#' reads.
#' @author Oscar Flores \email{oflores@@mmb.pcb.ub.es}
#' @seealso [ShortRead::AlignedRead], [GenomicRanges::GRanges],
#'   [fragmentLenDetect()]
#' @keywords manip
#' @rdname processReads
#'
#' @examples
#' # Load data
#' data(nucleosome_htseq)
#'
#' # Process nucleosome reads, select only those shorter than 200bp
#' pr1 <- processReads(nucleosome_htseq, fragmentLen=200)
#'
#' # Now process them, but picking only the 40 bases surrounding the dyad
#' pr2 <- processReads(nucleosome_htseq, fragmentLen=200, trim=40)
#'
#' # Compare the results:
#' library(ggplot2)
#' cov1 <- as.vector(coverage.rpm(pr1)[["chr1"]])
#' cov2 <- as.vector(coverage.rpm(pr2)[["chr1"]])
#' plot_data <- rbind(
#'     data.frame(x=seq_along(cov1), y=cov1, coverage="original"),
#'     data.frame(x=seq_along(cov2), y=cov2, coverage="trimmed")
#' )
#' qplot(x=x, y=y, geom="line", data=plot_data, xlab="position",
#'   ylab="coverage") + facet_grid(coverage~.)
#'
#' @export
#'
setGeneric(
    "processReads",
    function(data, type="single", fragmentLen, trim, ...)
        standardGeneric("processReads")
)

#' @rdname processReads
#' @importMethodsFrom BiocGenerics strand
#' @importFrom grDevices rgb
#' @importMethodsFrom Biostrings nchar
#' @importMethodsFrom ShortRead position sread chromosome
setMethod(
    "processReads",
    signature(data="AlignedRead"),
    function (data, type="single", fragmentLen, trim, ...) {

        # require("ShortRead")
        if (missing(fragmentLen)) {
            if (type == "single") {
                message(
                    " * fragmentLen not provided for strand correction,",
                    "infering automatically..."
                )
                fragmentLen <- fragmentLenDetect(data, ...)
                message(paste(" * fragmentLen =", fragmentLen))
            } else {
                fragmentLen <- Inf  # Don't remove anything
            }
        }

        #######################################################################
        if (type == "single") {  # Special case for trim==fragmentLength
            if (!missing(trim)) {
                if (trim == fragmentLen) {
                    start <- position(data)
                    neg.strand <- start + nchar(sread(data))
                    sel <- strand(data) == "-"
                    start[sel] <- neg.strand[sel]
                    res <- GRanges(
                        ranges   = IRanges(start=start, width=fragmentLen),
                        seqnames = chromosome(data)
                    )
                    return (res)
                }
            }

            # If no trim restriction, use whole read length,
            # else use trim length
            sr_len <- nchar(sread(data))

            if (!missing(trim)) {
                sr_len <- trim
            }

            # Floor, then we will add 1 if needed
            shift  <- floor((fragmentLen - sr_len) / 2)
            strand <- strand(data)

            new_levels <- vector(mode="integer", length=3)
            new_levels[which(levels(strand) == "-")] <- -1
            new_levels[which(levels(strand) == "+")] <- +1
            new_levels[which(levels(strand) == "*")] <- 0
            levels(strand) <- new_levels

            strand <- as.numeric(levels(strand))[strand]

            # Correction for non rounded shifts in negative strand (ie 147/40)
            # If the fragment_length - read_length is odd and negative strand,
            # add 1 extra base
            correct <- rep(0, length(shift))
            sel <- (strand == -1) & ((fragmentLen-sr_len) %% 2 == 1)
            correct[sel] <- 1

            start <- position(data) + ((shift+correct) * strand)
            res <- GRanges(
                ranges = IRanges(start=start, width=sr_len),
                space  = chromosome(data)
            )

        #######################################################################
        } else if (type == "paired") {
            sr_len <- nchar(sread(data))
            selection <- sr_len < fragmentLen
            seqnames <- chromosome(data)[selection]

            # Normal start or trimmed version if requested
            start <- position(data)[selection]
            if (!missing(trim)) {
                start <- start + (sr_len[selection] / 2 - floor(trim / 2))
            }

            width <- sr_len[selection]
            if (!missing(trim)) {
                width <- trim
            }
            res <- GRanges(
                ranges   = IRanges(start=start, width=width),
                seqnames = seqnames
            )

        #######################################################################
        } else {
            stop(paste(
                "type must be 'single' for single-ended data or 'paired' ",
                "for paired-ended"
            ))
        }

        return (res)
    }
)

#' @rdname processReads
#' @importFrom GenomicRanges GRangesList
setMethod(
    "processReads",
    signature(data="CompressedGRangesList"),
    function (data, type="single", fragmentLen, trim, ...)
        GRangesList(lapply(data,
                           processReads,
                           type=type,
                           fragmentLen=fragmentLen,
                           trim=trim,
                           ...))
)

#' @rdname processReads
#' @importFrom GenomicRanges GRanges
#' @importMethodsFrom BiocGenerics end
#' @importMethodsFrom GenomeInfoDb seqnames
#' @importMethodsFrom S4Vectors space
setMethod(
    "processReads",
    signature(data="GRanges"),
    function (data, type="single", fragmentLen, trim, ...) {

        if (missing(fragmentLen)) {
            if (type == "single") {
                message(
                    " * fragmentLen not provided for strand correction, ",
                    "infering automatically..."
                )
                fragmentLen <- fragmentLenDetect(data, ...)
                message(paste(" * fragmentLen =", fragmentLen))
            } else {
                fragmentLen <- Inf  # Don't remove anything
            }
        }

        #######################################################################
        if (type == "single") {  # Special case for trim==fragmentLength
            if (!missing(trim)) {
                if (trim == fragmentLen) {
                    start <- start(data)
                    negStrand <- data$strand == "-"
                    start[negStrand] <- end(data)[negStrand] - fragmentLen
                    res <- GRanges(
                        ranges   = IRanges(start=start, width=fragmentLen),
                        seqnames = seqnames(data)
                    )
                    return (res)
                }
            }

            # If no trim restriction, use whole read length,
            # else use trim length
            if (missing(trim)) {
                sr_len <- width(data)
            } else {
                sr_len <- trim
            }

            # Floor, then we will add 1 if needed
            shift <- floor((fragmentLen - sr_len) / 2)

            strand <- rep(1, length(data))
            strand[data$strand == "-"] <- -1

            # Correction for non rounded shifts in negative strand (ie 147/40)
            # If the fragment_length - read_length is odd and negative strand,
            # add 1 extra base
            correct <- rep(0, length(strand))
            correct[(strand == -1) & ((fragmentLen - sr_len) %% 2 == 1)] <- 1

            start <- start(data) + ((shift+correct) * strand)

            res <- GRanges(
                ranges   = IRanges(start=start, width=sr_len),
                seqnames= seqnames(data)
            )

        #######################################################################
        } else if (type == "paired") {
            data <- data[width(data) < fragmentLen, ]
            sr_len <- width(data)

            # Normal start or trimmed version if requested
            start <- start(data)
            if (!missing(trim)) {
                start <- start + ((sr_len / 2) - floor(trim / 2))
            }

            width <- sr_len

            if (!missing(trim)) {
                width <- trim
            }

            res <- GRanges(
                ranges   = IRanges(start=start, width=width),
                seqnames = seqnames(data)
            )
        #######################################################################
        } else {
            stop(paste(
                "type must be 'single' for single-ended data or",
                "'paired' for paired-ended"
            ))
        }

        return (res)
    }
)
nucleosome-dynamics/nucleR documentation built on May 6, 2021, 4:47 p.m.