ThreeConditions: Intermediates and Results from Cell Tracking Analyses

In ocbe-uio/cellmigRation: Track Cells, Analyze Cell Trajectories and Compute Migration Statistics

Description

Intermediates and Results from Cell Tracking Analyses, used to build the package vignette.

Usage

data(ThreeConditions)

Format

a list including 3 elements

Details

BT549 cell trajectories were computed using cellmigRation. Imaging experiments were performed as described by Ghannoum S et al (paper in preparation). Briefly, triple negative breast cancer BT549 cells were cultured in RPMI supplemented with 10 and 1 NucLight green lentivirus (Essen BioScience), and then sorted by fluorescence-activated cell sorting (FACS). GFP-positive cells were seeded at a 1:3 ratio with untransduced BT549 cells in 96-well image-lock plates (EssenBio) at a density of 1000 total cells per well. Cells were scanned at ten-minute intervals over 24h using an Incucyte S3 Live-Cell microscope (EssenBio) at 10x magnification and a Basler Ace 1920-155um camera with CMOS sensor. Cells were treated with 100 uM Rac1 Inhibitor (1177865-17-6, Calbiochem) or left untreated (controls). TIFF images were imported and processed using the cellmigRation library.

Examples

data(ThreeConditions)

ocbe-uio/cellmigRation documentation built on Dec. 16, 2021, 10:59 p.m.