WSADataset: Trajectories of 147 cells

In ocbe-uio/cellmigRation: Track Cells, Analyze Cell Trajectories and Compute Migration Statistics

Description

A dataset containing the coordinates and the ID of 147 cells from wound scratch migration experiment

Usage

data(WSADataset)

Format

A data frame with 11970 rows and 4 columns

Details

BT549 cell trajectories were computed using cellmigRation. Imaging experiments were performed as described by Ghannoum S et al (paper in preparation). Briefly, triple negative breast cancer BT549 cells were cultured in RPMI supplemented with 10 and 1 NucLight green lentivirus (Essen BioScience), and then sorted by fluorescence-activated cell sorting (FACS). GFP-positive cells were seeded at a 1:3 ratio with untransduced BT549 cells in 96-well image-lock plates (EssenBio) at a density of 1000 total cells per well. Once cells reached the desired density, a thin wound was introduced by scratching the cell monolayer. Next, cells were scanned at ten-minute intervals over 24h using an Incucyte S3 Live-Cell microscope (EssenBio) at 10x magnification and a Basler Ace 1920-155um camera with CMOS sensor. TIFF images were imported and processed using the cellmigRation library.

Examples

data(WSADataset)

ocbe-uio/cellmigRation documentation built on Dec. 16, 2021, 10:59 p.m.