countReads: countReads

Usage Arguments

View source: R/countReads.R

Usage

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countReads(samplenames, gtf, fragmentLength = 150, bamFolder,
  outputDir = NA, modification = "m6A", binSize = 50,
  strandToKeep = "opposite", paired = FALSE, threads = 1)

Arguments

samplenames

The names of each sample (prefix for bam files)

gtf

The gtf format gene annotation file

fragmentLength

The RNA fragment length (insert size of the library).

bamFolder

Path to the folder where bam file locates

outputDir

The directory to save output files

modification

The modification used to name the BAM files.

binSize

The size of consecutive bins to slice the transcripts

strandToKeep

According to library preparation protocol, choose which strand to count. Stranded RNA library usually seq the "ooposite" strand. Small RNA library seq the "same" strand.

paired

Logical indicating whether the input bam files are from paired end sequencing. Default is FALSE. If using paired end data, the read length will be estimated from the data and only good mate are counted.

threads

The number of threads to use for hyperthreading

sasaveOutput

Logical option indicating whether to save output as an RDS file.


scottzijiezhang/m6Amonster documentation built on Jan. 8, 2021, 1:37 p.m.