proteome: Proteome

Description Usage Arguments Examples

View source: R/proteome.R

Description

Creates a data frame with an average abundance of the top ionizers for each protein

Usage

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proteome(file_name, organism, wt_samps = "all", group = FALSE,
  group_names = NULL, top_pep = 3, min_pep = 3, norm = FALSE,
  norm_method = NULL, norm_pro = NULL, csv = TRUE)

Arguments

file_name

file containing all peptides for all samples with the columns named Annotated.Sequence, Modifications, Master.Protein.Accessions.

organism

"mouse", "human" or "rat"

wt_samps

"all" if you want to use all samples to weight, otherwise use a pattern that is present in the column names of the samples you wish to use for the weighting

group

if you want to group samples then TRUE

group_names

indicate names of the groups (must be a string pattern present in the samples)

top_pep

number of top ionizers used

min_pep

minumum number of peptides found in a protein to be incuded in the analysis

norm

set to TRUE if normalization is wanted

norm_method

indicate normalization method: "protein" or "sum_total"

norm_pro

accession of protein used for

csv

if result file should be written in working directory

Examples

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proteome("WT vs KO all pep.csv", group_names = c("WT", "KO"),
         organism = "mouse", group = TRUE, norm = TRUE,
         norm_method = "protein", norm_pro = "B2RTM0", csv = FALSE)

tsoleary/proteomixr documentation built on July 19, 2019, 8:37 a.m.