differential_edgeR_pseudobulk_LRT.Seurat: Pseudo-bulk differential analysis with edgeR (LRT)

In vallotlab/IDclust: Iterative Differential Clustering for single-cell

Pseudo-bulk differential analysis with edgeR (LRT)

Description

Pseudo-bulk differential analysis with edgeR (LRT)

Usage

## S3 method for class 'Seurat'
differential_edgeR_pseudobulk_LRT(
  object,
  by = "IDcluster",
  assay = "RNA",
  biological_replicate_col = NULL,
  logFC.th = log2(2),
  qval.th = 0.01,
  min.pct = 0.2
)

Arguments

object	A Seurat object containing scRNA dataset with a metadata column name matching the by parameter
by	A character specifying the name of the metadata column referencing the clusters.
assay	Assay to use.
biological_replicate_col	A character specifying the column of the object metadata definin the biological / technical replicates. If NULL, three random set of replicates will be created per cluster, provided there are enough cells.
logFC.th	A numeric specifying the log2 fold change of activation above/below which a feature is considered as significantly differential.
qval.th	A numeric specifying the adjusted p-value below which a feature is considered as significantly differential.
min.pct	Minimum percentage of cells to be active in the cells of the cluster to consider a feature as potentially significantly differential.

Details

Concatenate single-cells into replicates by cluster in order to create a 'pseudo-bulk' matrice of multiple replicates per cluster. If no replicates are present, will assign replicates at random to create 3 replicates per cluster. Conducts 'LRT' (likelihood ratio tests) edgeR tests to test. See edgeR::glmLRT()

Value

A data.frame containing the results of the differential analysis

See Also

See edgeR::glmLRT()

Examples

if(requireNamespace("Seurat", quietly=TRUE)){
data("Seu", package = "IDclust")
DA <- differential_edgeR_pseudobulk_LRT(Seu)
}

vallotlab/IDclust documentation built on July 5, 2024, 3:26 p.m.