read.qPCR: Reads in qRT-PCR file
In wjawaid/bglab: Berthold Gottgens lab scRNA seq

Description Usage Arguments Details Value Author(s)

Reads in file Reads in the qRT-PCR file. A tab delimited file containing -δCT qRT-PCR data

1 2	read.qPCR(file, exclude = NULL, sep = "\t", cellIDcol = 1, removeCol = NULL, cellStageCol = NULL)

`file`	Path of file and name containing -δCT qRT-PCR data
`exclude`	Include names of genes that should be excluded from the analysis e.g. housekeeping genes that were used to normalize the data or for those that did not work well
`sep`	Separator used in file containing qPCR data
`cellIDcol`	Column containing the cell ID to be used
`removeCol`	Unnecessary columns to be removed
`cellStageCol`	Column containing cell stage data

Reads in the qRT-PCR file. A tab delimited file containing normalized -δCT qRT-PCR data, assuming the first line is a header containing gene names and the first coloumn contains cell identifiers.

A matrix where each row represents a cell and each coloumn a gene

Wajid Jawaid

wjawaid/bglab documentation built on May 4, 2019, 6:33 a.m.