filterExtremePeakGenes: Filter out transcript by a median filter

Description Usage Arguments Value

View source: R/coverage_filters.R

Description

For removing very extreme peaks in coverage plots, use high quantiles, like 99. Used to make your plots look better, by removing extreme peaks.

Usage

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filterExtremePeakGenes(
  tx,
  reads,
  upstream = NULL,
  downstream = NULL,
  multiplier = "0.99",
  min_cutoff = "0.999",
  pre_filter_minimum = 0,
  average = "median"
)

Arguments

tx

a GRangesList

reads

a GAlignments or GRanges

upstream

numeric or NULL, default NULL. if you want window of tx, instead of whole, specify how much upstream from start of tx, 10 is include 10 bases before start

downstream

numeric or NULL, default NULL. if you want window of tx, instead of whole, specify how much downstream from start of tx, 10 is go 10 bases into tx from start.

multiplier

a character or numeric, default "0.99", either a quantile if input is string[0-1], like "0.99", or numeric value if input is numeric. How much bigger than median / mean counts per gene, must a value be to be defined as extreme ?

min_cutoff

a character or numeric, default "0.999", either a quantile if input is string[0-1], like "0.999", or numeric value if input is numeric. Lowest allowed value

pre_filter_minimum

numeric, default 0. If value is x, will remove all positions in all genes with coverage < x, before median filter is applied. Set to 1 to remove all 0 positions.

average

character, default "median". Alternative: "mean". How to scale the multiplier argument, from median or mean of gene coverage.

Value

GRangesList (filtered)


ORFik documentation built on March 27, 2021, 6 p.m.