filterVcfMuTect2: Filter VCF MuTect2

In PureCN: Copy number calling and SNV classification using targeted short read sequencing

Description

Function to remove artifacts and low confidence/quality calls from a GATK4/MuTect2 generated VCF file. Also applies filters defined in filterVcfBasic.

Usage

filterVcfMuTect2(
  vcf,
  tumor.id.in.vcf = NULL,
  ignore = c("clustered_events", "t_lod", "str_contraction", "read_position",
    "position", "fragment_length", "multiallelic", "clipping", "strand_artifact",
    "strand_bias", "slippage", "weak_evidence", "orientation", "haplotype"),
  ...
)

Arguments

vcf	CollapsedVCF object, read in with the readVcf function from the VariantAnnotation package.
tumor.id.in.vcf	The tumor id in the VCF file, optional.
ignore	MuTect2 flags that mark variants for exclusion.
...	Additional arguments passed to filterVcfBasic.

Value

A list with elements vcf, flag and flag_comment. vcf contains the filtered CollapsedVCF, flag a logical(1) flag if problems were identified, further described in flag_comment.

Author(s)

Markus Riester

See Also

filterVcfBasic

Examples

### This function is typically only called by runAbsolute via the 
### fun.filterVcf and args.filterVcf comments.
library(VariantAnnotation)    
vcf.file <- system.file("extdata", "example.vcf.gz", package="PureCN")
vcf <- readVcf(vcf.file, "hg19")
vcf.filtered <- filterVcfMuTect(vcf)        

PureCN documentation built on Nov. 8, 2020, 5:37 p.m.