peakScoring: Peak scoring function

Description Usage Arguments Details Value Author(s) See Also Examples

Description

Scores peaks detected with function peakDetection according the height and the sharpness (width) of the peak. This function can be called automatically from peakDetection if score=TRUE.

Usage

 1
 2
 3
 4
 5
 6
 7
 8
 9
10
11
12
13
14
15
16
17
peakScoring(peaks, data, threshold = 0.25, ...)

## S4 method for signature 'list'
peakScoring(peaks, data, threshold = "25%", mc.cores = 1)

## S4 method for signature 'IRangesList'
peakScoring(peaks, data, threshold = "25%",
  weight.width = 1, weight.height = 1, dyad.length = 38, mc.cores = 1)

## S4 method for signature 'numeric'
peakScoring(peaks, data, chromosome = NULL,
  threshold = "25%")

## S4 method for signature 'IRanges'
peakScoring(peaks, data, chromosome = NULL,
  threshold = "25%", weight.width = 1, weight.height = 1,
  dyad.length = 38)

Arguments

peaks

The identified peaks resulting from peakDetection. Could be a numeric vector with the position of the peaks, or a IRanges object with the extended range of the peak. For both types, list support is implemented as a numeric list or a IRangesList

data

Data of nucleosome coverage or intensites.

threshold

The non-default threshold previously used in peakDetection function, if applicable. Can be given as a percentage string (i.e., "25\%" will use the value in the 1st quantile of data) or as an absolute coverage numeric value (i.e., 20 will not look for peaks in regions without less than 20 reads (or reads per million)).

...

Further arguments to be passed to or from other methods.

mc.cores

If input is a list or IRangeList, and multiple cores support is available, the maximum number of cores for parallel processing.

weight.height, weight.width

If the score is a range, the height and the widht score (coverage and fuzzynes) can be defined with different weigths with these parameters. See details.

dyad.length

How many bases account in the nucleosome dyad for sharpness description. If working with NGS data, works best with the reads width value for single-ended data or the trim value given to the processReads function.

chromosome

Optionally specify the name of the chromosome for input data that doesn't specify it.

Details

This function scores each previously identified peak according its height and sharpness.

The height score (score_h) tells how large is a peak, higher means more coverage or intensity, so better positioned nucleosome. This score is obtained by checking the observed peak value in a Normal distribution with the mean and sd of data. This value is between 0 and 1.

The width score (score_w) is a mesure of how sharp is a peak. With a NGS coverage in mind, a perfect phased (well-positioned) nucleosome is this that starts and ends exactly in the same place many times. The shape of this ideal peak will be a rectangular shape of the lenght of the read. A wider top of a peak could indicate fuzzyness. The parameter dyad.length tells how long should be the "flat" region of an ideal peak. The optimum value for this parameter is the lenght of the read in single-ended data or the trim value of the function processReads. For Tiling Arrays, the default value should be fine.

This score is obtained calculating the ratio between the mean of the nucleosome scope (the one provided by range in the elements of peaks) and the dyad.length central bases. This value is normalized between 0 and 1.

For punctual, single points peaks (provided by numeric vector or list as peaks attribute) the score returned is the height score.

For range peaks the weighted sum of the heigth and width scores is used. This is: ((score_h * weigth.height) / sum.wei) + ((score_w * weigth.width) / sum.wei). Note that you can query for only one score by setting its weight to 1 and the other to 0.

Value

In the case of numeric input, the value returned is a data.frame containing a 'peak' and a 'score' column. If the input is a list, the result will be a list of data.frame.

If input is a IRanges or IRangesList, the result will be a data.frame or GenomicRanges::GRanges object with one or multiple spaces respectively and a 3 data column with the mixed, width and heigh score.

Author(s)

Oscar Flores [email protected]

See Also

peakDetection(), processReads(),

Examples

 1
 2
 3
 4
 5
 6
 7
 8
 9
10
11
12
# Generate a synthetic map

# Trimmed length nucleosome map
map <- syntheticNucMap(nuc.len=40, lin.len=130)

# Get the information of dyads and the coverage
peaks <- c(map$wp.starts, map$fz.starts)
cover <- filterFFT(coverage.rpm(map$syn.reads))

# Calculate the scores
scores <- peakScoring(peaks, cover)
plotPeaks(scores$peak, cover, scores=scores$score, start=5000, end=10000)

nucleR documentation built on Nov. 1, 2018, 2:23 a.m.