getPvals: Estimate the empirical p-values

Description Usage Arguments Value Examples

View source: R/permuTest.R

Description

With null distributions estimated using the generateNull() function, p-values are estimated using a one-tailed test. A minimum p-value of 1/B can be achieved with B permutations.

Usage

1
getPvals(permuteResult, scoredf, subSamples = NULL)

Arguments

permuteResult

A matrix, null distributions for each sample generated using the generateNull() function

scoredf

A dataframe, the scored results of samples under test generated using the simpleScore() function

subSamples

A vector of sample labels/indices that will be used to subset the score matrix. All samples will be scored if not provided

Value

Estimated p-values for enrichment of the signature in each sample. A p-value of 1/B indicates that the estimated p-value is less than or equal to 1/B.

Examples

 1
 2
 3
 4
 5
 6
 7
 8
 9
10
11
12
ranked <- rankGenes(toy_expr_se)
scoredf <- simpleScore(ranked, upSet = toy_gs_up, downSet = toy_gs_dn)
# find out what backends can be registered on your machine
BiocParallel::registered()
# the first one is the default backend, and it can be changed explicitly.
# See vignette for more details
permuteResult = generateNull(upSet = toy_gs_up, downSet = toy_gs_dn, ranked,
B =10, seed = 1, useBPPARAM = NULL)

# call the permutation function to generate the empirical scores
# for B times.
pvals <- getPvals(permuteResult,scoredf)

singscore documentation built on April 29, 2020, 5:18 a.m.