Example10x: Small example 10x Genomics Ig V(D)J sequences from CD19+ B...

In alakazam: Immunoglobulin Clonal Lineage and Diversity Analysis

Small example 10x Genomics Ig V(D)J sequences from CD19+ B cells isolated from PBMCs of a healthy human donor. Down-sampled from data provided by 10x Genomics under a Creative Commons Attribute license, and processed with their Cell Ranger pipeline.

Description

Small example 10x Genomics Ig V(D)J sequences from CD19+ B cells isolated from PBMCs of a healthy human donor. Down-sampled from data provided by 10x Genomics under a Creative Commons Attribute license, and processed with their Cell Ranger pipeline.

Usage

Example10x

Format

A data.frame with the following AIRR style columns:

• sequence_id: Sequence identifier

• sequence_alignment: IMGT-gapped observed sequence.

• germline_alignment: IMGT-gapped germline sequence.

• v_call: V region allele assignments.

• d_call: D region allele assignments.

• j_call: J region allele assignments.

• c_call: Isotype (C region) assignment.

• junction: Junction region sequence.

• junction_length: Length of the junction region in nucleotides.

• np1_length: Combined length of the N and P regions proximal to the V region.

• np2_length: Combined length of the N and P regions proximal to the J region.

• umi_count: Number of unique molecular identifies atttributed to sequence.

• cell_id: Cell identifier.

• locus: Genomic locus of sequence.

References

1. Data source: https://support.10xgenomics.com/single-cell-vdj/datasets/2.2.0/vdj_v1_hs_cd19_b

2. License: https://creativecommons.org/licenses/by/4.0/

alakazam documentation built on Sept. 30, 2023, 9:07 a.m.