ddpcRquant: Quantify droplets
In dpcR: Digital PCR Analysis
Description
Usage
Arguments
Value
Note
Author(s)
References
Description
Cluster raw data from QX100 and QX200 systems.
Usage
1
2
ddpcRquant(path, threshold.int = 0.9995, reps = 10, blocks = 150,
threshold.manual = NULL)
Arguments
path
character path to diectory with raw data.
threshold.int
numeric probability of the threshold quantile.
reps
numeric vector representing the number of replications.
blocks
numeric vector representing the number of blocks.
threshold.manual
if numeric, the value is used as the threshold. If
NULL, the threshold is calculated automatically.
Value
dpcr object.
Note
This function is a modification of code was implemented using the code found on
http://www.ddpcrquant.ugent.be/.
Author(s)
Wim Trypsteen, Matthijs Vynck, Jan De Neve, Pawel Bonczkowski, Maja Kiselinova,
Eva Malatinkova, Karen Vervisch, Olivier Thas, Linos Vandekerckhove, Ward De Spiegelaere.
References
Trypsteen W, Vynck M, De Neve J, Bonczkowski P, Kiselinova M,
Malatinkova E, Vervisch K, Thas O, Vandekerckhove L, De Spiegelaere W,
ddpcRquant: Threshold Determination for Single Channel Droplet Digital PCR
Experiments. Analytical and Bioanalytical Chemistry 2015. 407(19): p.5827-34.
dpcR documentation built on May 2, 2019, 7:04 a.m.
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Description Usage Arguments Value Note Author(s) References
Description
Cluster raw data from QX100 and QX200 systems.
Usage
1 2 | ddpcRquant(path, threshold.int = 0.9995, reps = 10, blocks = 150,
threshold.manual = NULL)
|
Arguments
path |
|
threshold.int |
|
reps |
|
blocks |
|
threshold.manual |
if |
Value
dpcr object.
Note
This function is a modification of code was implemented using the code found on http://www.ddpcrquant.ugent.be/.
Author(s)
Wim Trypsteen, Matthijs Vynck, Jan De Neve, Pawel Bonczkowski, Maja Kiselinova, Eva Malatinkova, Karen Vervisch, Olivier Thas, Linos Vandekerckhove, Ward De Spiegelaere.
References
Trypsteen W, Vynck M, De Neve J, Bonczkowski P, Kiselinova M, Malatinkova E, Vervisch K, Thas O, Vandekerckhove L, De Spiegelaere W, ddpcRquant: Threshold Determination for Single Channel Droplet Digital PCR Experiments. Analytical and Bioanalytical Chemistry 2015. 407(19): p.5827-34.
R Package Documentation
Browse R Packages
We want your feedback!
Note that we can't provide technical support on individual packages. You should contact the package authors for that.
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