df2dpcr: Convert data.frame to dpcr object
In dpcR: Digital PCR Analysis

Description Usage Arguments Details Value Author(s) See Also Examples

Converts data.frame object to to adpcr or dpcr object. The resulting object will have "tnp" type.

1	df2dpcr(df)

`df`	data frame with specified column names. See Details.

The data frame must have REDF structure. It means that data must contain following columns with exactly specified names:

experiment: names of experiments
replicate: indices of replicates
assay: names of assays
k: number of positive partitions
n: total number of partitions
v: volume of partition (nL)
uv: uncertainty of partition's volume (nL)
threshold: partitions with k equal or higher than threshold are treated as positve.

There are also one optional column:

panel_id: indices of panels

If the additional column is present, the resulting object has adpcr type.

An object of adpcr or dpcr type, depends on the presence of additional column with panel indices (see Details).

Michal Burdukiewcz, Stefan Roediger

Flexibly create dpcr objects: create_dpcr Inverse function: dpcr2df

dat <- data.frame(experiment = factor(rep(paste0("Experiment", 1L:2), 3)),
                  replicate = c(1, 1, 2, 2, 3, 3),
                  assay = "Assay1",
                  k = c(55, 121, 43, 150, 70, 131),
                  n = 765,
                  v = 1,
                  uv = 0)
df2dpcr(dat)