solve_pbtk: Solve_PBTK

In httk: High-Throughput Toxicokinetics

Solve_PBTK

Description

This function solves for the amounts or concentrations in uM of a chemical in different tissues as functions of time based on the dose and dosing frequency. In this PBTK formulation. C_{tissue} is the concentration in tissue at time t. Since the perfusion limited partition coefficients describe instantaneous equilibrium between the tissue and the free fraction in plasma, the whole plasma concentration is C_{tissue,plasma} = \frac{1}{f_{up}*K_{tissue2fup}}*C_{tissue}. Note that we use a single, constant value of f_{up} across all tissues. Corespondingly the free plasma concentration is modeled as C_{tissue,free plasma} = \frac{1}{K_{tissue2fup}}*C_tissue. The amount of blood flowing from tissue x is Q_{tissue} (L/h) at a concentration C_{x,blood} = \frac{R_{b2p}}{f_{up}*K_{tissue2fup}}*C_{tissue}, where we use a single R_{b2p} value throughout the body. Metabolic clearance is modelled as being from the total plasma concentration here, though it is restricted to the free fraction in calc_hep_clearance by default. Renal clearance via glomerulsr filtration is from the free plasma concentration. The compartments used in this model are the gutlumen, gut, liver, kidneys, veins, arteries, lungs, and the rest of the body. The extra compartments include the amounts or concentrations metabolized by the liver and excreted by the kidneys through the tubules. AUC is the area under the curve of the plasma concentration.

Usage

solve_pbtk(
  chem.name = NULL,
  chem.cas = NULL,
  dtxsid = NULL,
  times = NULL,
  parameters = NULL,
  days = 10,
  tsteps = 4,
  daily.dose = NULL,
  dose = NULL,
  doses.per.day = NULL,
  initial.values = NULL,
  plots = FALSE,
  suppress.messages = FALSE,
  species = "Human",
  iv.dose = FALSE,
  input.units = "mg/kg",
  output.units = NULL,
  default.to.human = FALSE,
  class.exclude = TRUE,
  recalc.blood2plasma = FALSE,
  recalc.clearance = FALSE,
  dosing.matrix = NULL,
  adjusted.Funbound.plasma = TRUE,
  regression = TRUE,
  restrictive.clearance = TRUE,
  minimum.Funbound.plasma = 1e-04,
  Caco2.options = list(),
  monitor.vars = NULL,
  ...
)

Arguments

chem.name	Either the chemical name, CAS number, or the parameters must be specified.
chem.cas	Either the chemical name, CAS number, or the parameters must be specified.
dtxsid	EPA's DSSTox Structure ID (https://comptox.epa.gov/dashboard) the chemical must be identified by either CAS, name, or DTXSIDs
times	Optional time sequence for specified number of days. Dosing sequence begins at the beginning of times.
parameters	Chemical parameters from parameterize_pbtk function, overrides chem.name and chem.cas.
days	Length of the simulation.
tsteps	The number of time steps per hour.
daily.dose	Total daily dose, defaults to mg/kg BW.
dose	Amount of a single, initial oral dose in mg/kg BW.
doses.per.day	Number of doses per day.
initial.values	Vector containing the initial concentrations or amounts of the chemical in specified tissues with units corresponding to output.units. Defaults are zero.
plots	Plots all outputs if true.
suppress.messages	Whether or not the output message is suppressed.
species	Species desired (either "Rat", "Rabbit", "Dog", "Mouse", or default "Human").
iv.dose	Simulates a single i.v. dose if true.
input.units	Input units of interest assigned to dosing, defaults to mg/kg BW
output.units	A named vector of output units expected for the model results. Default, NULL, returns model results in units specified in the 'modelinfo' file. See table below for details.
default.to.human	Substitutes missing animal values with human values if true (hepatic intrinsic clearance or fraction of unbound plasma).
class.exclude	Exclude chemical classes identified as outside of domain of applicability by relevant modelinfo_[MODEL] file (default TRUE).
recalc.blood2plasma	Recalculates the ratio of the amount of chemical in the blood to plasma using the input parameters, calculated with hematocrit, Funbound.plasma, and Krbc2pu.
recalc.clearance	Recalculates the the hepatic clearance (Clmetabolism) with new million.cells.per.gliver parameter.
dosing.matrix	Vector of dosing times or a matrix consisting of two columns or rows named "dose" and "time" containing the time and amount, in mg/kg BW, of each dose.
adjusted.Funbound.plasma	Uses adjusted Funbound.plasma when set to TRUE along with partition coefficients calculated with this value.
regression	Whether or not to use the regressions in calculating partition coefficients.
restrictive.clearance	Protein binding not taken into account (set to 1) in liver clearance if FALSE.
minimum.Funbound.plasma	Monte Carlo draws less than this value are set equal to this value (default is 0.0001 – half the lowest measured Fup in our dataset).
Caco2.options	A list of options to use when working with Caco2 apical to basolateral data Caco2.Pab, default is Caco2.options = list(Caco2.Pab.default = 1.6, Caco2.Fabs = TRUE, Caco2.Fgut = TRUE, overwrite.invivo = FALSE, keepit100 = FALSE). Caco2.Pab.default sets the default value for Caco2.Pab if Caco2.Pab is unavailable. Caco2.Fabs = TRUE uses Caco2.Pab to calculate fabs.oral, otherwise fabs.oral = Fabs. Caco2.Fgut = TRUE uses Caco2.Pab to calculate fgut.oral, otherwise fgut.oral = Fgut. overwrite.invivo = TRUE overwrites Fabs and Fgut in vivo values from literature with Caco2 derived values if available. keepit100 = TRUE overwrites Fabs and Fgut with 1 (i.e. 100 percent) regardless of other settings. See get_fbio for further details.
monitor.vars	Which variables are returned as a function of time. The default value of NULL provides "Cgut", "Cliver", "Cven", "Clung", "Cart", "Crest", "Ckidney", "Cplasma", "Atubules", "Ametabolized", and "AUC"
...	Additional arguments passed to the integrator (deSolve).

Details

Note that the model parameters have units of hours while the model output is in days.

Default NULL value for doses.per.day solves for a single dose.

Model Figure

When species is specified as rabbit, dog, or mouse, the function uses the appropriate physiological data(volumes and flows) but substitutes human fraction unbound, partition coefficients, and intrinsic hepatic clearance.

Value

A matrix of class deSolve with a column for time(in days), each compartment, the area under the curve, and plasma concentration and a row for each time point.

Author(s)

John Wambaugh and Robert Pearce

References

\insertRef

pearce2017httkhttk

See Also

solve_model

parameterize_gas_pbtk

calc_analytic_css_pbtk

Examples

# Multiple doses per day:
head(solve_pbtk(
  chem.name='Bisphenol-A',
  daily.dose=.5,
  days=2.5,
  doses.per.day=2,
  tsteps=2))

# Starting with an initial concentration:
out <- solve_pbtk(
  chem.name='bisphenola',
  dose=0,
  days=2.5,
  output.units="mg/L", 
  initial.values=c(Agut=200))

# Working with parameters (rather than having solve_pbtk retrieve them):
params <- parameterize_pbtk(chem.cas="80-05-7")
head(solve_pbtk(parameters=params, days=2.5))
                  
# We can change the parameters given to us by parameterize_pbtk:
params <- parameterize_pbtk(dtxsid="DTXSID4020406", species = "rat")
params["Funbound.plasma"] <- 0.1
out <- solve_pbtk(parameters=params, days=2.5)

# A fifty day simulation:
out <- solve_pbtk(
  chem.name = "Bisphenol A", 
  days = 50, 
  daily.dose=1,
  doses.per.day = 3)
plot.data <- as.data.frame(out)
css <- calc_analytic_css(chem.name = "Bisphenol A")

library("ggplot2")
c.vs.t <- ggplot(plot.data, aes(time, Cplasma)) + 
  geom_line() +
  geom_hline(yintercept = css) + 
  ylab("Plasma Concentration (uM)") +
  xlab("Day") + 
  theme(
    axis.text = element_text(size = 16), 
    axis.title = element_text(size = 16), 
    plot.title = element_text(size = 17)) +
  ggtitle("Bisphenol A")
print(c.vs.t)

httk documentation built on Sept. 11, 2024, 9:32 p.m.