ebseq: Function "ebseq"

Description Usage Arguments Value

View source: R/ebseq.R

Description

This function Perform Differential Gene Expression Analysis using EBSeq.

Usage

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ebseq(type, data, condition, n1, n2, N = 5, fdr = 0.05,
  method = "robust", fdrmethod = "hard", fc = 0.7, Qcut = 0,
  tt = 3)

Arguments

type

a character type variable indicating whether to perform "Gene-level analysis" or "Isoform-level analysis".

data

either an unnormalized counts matrix for "Gene-level analysis", or a list for "Isoform-level analysis".

condition

a length two variable containing two conditions.

n1

an integer indicating the number of samples in the first condition.

n2

an integer indicating the number of samples in the second condition.

N

an integer indicating the number of iterations in EB Test.

fdr

a numeric variable indicating the target FDR in EB Test.

method

"robust" or "classic". Refer to GetDEResults() in EBSeq. Default is "robust".

fdrmethod

"hard" or "soft". Refer to GetDEResults() in EBSeq. Default is "hard".

fc

Threshold for the fold change statistics.Default is 0.7.

Qcut

Transcrips with 100 th quantile <= Qcut will be removed before testing. Default is 0.

tt

The number of uncertainty groups the user wish to define. Default is 3.

Value

a list consisting of the output and results from EB Test.


Coraline66/RNASeqAnalysis documentation built on Nov. 25, 2019, 8:03 a.m.