This package provides functions to analyze FRET binding data. Given raw fluorescence data from a FRET titration experiment's donor, acceptor and FRET channels, it allows to: (1) plot all channels to visually inspect raw data; (2) correct FRET signal; (3) estimate parameters of the quadratic binding equation (Kd, signal_min, signal_max); (4) fit the quadratic binding equation to the data; (5) report Kd; and (6) plot the corrected FRET and resulting binding curve.
Package details |
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Maintainer | |
License | MIT + file LICENSE |
Version | 0.0.0.9000 |
Package repository | View on GitHub |
Installation |
Install the latest version of this package by entering the following in R:
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