fp_calculate_pola_aniso_int: Calculate fluorescence polarization and anisotropy from raw...

In Guilz/rfret: Analyze FRET Binding Data

Description

This function calculates fluorescence polarization and anisotropy values from the raw data of parallel and perpendicularly polarized channels.

Usage

fp_calculate_pola_aniso_int(raw_data)

Arguments

raw_data

A dataframe containing the raw data. It must contain at least 7 columns named: Experiment A unique name identifying each experiment. Type For example, "titration". Replicate A number identifying the technical replicate (1, 2, etc.). Observation A number identifying each observation in a titration series (corresponds to the plate column numbers, if experiments are set up as rows in a 384-well plate). The number of observations for an experiment and its blanks must match, and a given observation number must associate data points at the same concentration in the titration series. concentration The ligand concentration in the titration series. parallel The name of the column containing fluorescence intensities from the parallel channel. perpendicular The name of the column containing fluorescence intensities from the perpendicular channel. The output of format_data can be used directly as input for this function.

Value

A dataframe containing the same columns as the input dataframe, plus three columns called polarization, anisotropy and intensity.

See Also

format_data to prepare datasets for use with fp_calculate_pola_aniso_int.

Guilz/rfret documentation built on Oct. 18, 2021, 2:14 p.m.