fret_binding_data: A FRET binding assay dataset
In Guilz/rfret: Analyze FRET Binding Data

Description Usage Format Source

This dataset was collected on June 2nd, 2017. It is a protein-DNA interaction assay, where the protein is labeled with a donor fluorophore and the DNA with an acceptor fluorophore. This dataset yields a binding constant of 4.3 +/- 0.5 (using the hyperbolic model).

1	fret_binding_data

A data frame with 144 rows and 7 columns. Rows contain two replicates of three titrations series, each containing 24 data points. Columns are:

Well Row: The row where the sample corresponding to this record was located into the 384-well microplate.
Well Col: The column where the sample corresponding to this record was located into the 384-well microplate.
Content: A word describing the experiment and replicate this particular data point belongs to (acceptor_only.1, acceptor_only.2, donor_only.1, donor_only.2 titration.1, titration.2).
fret_channel: The fluorescence intensities measured in the FRET channel.
acceptor_channel: The fluorescence intensities measured in the acceptor channel.
donor_channel: The fluorescence intensities measured in the donor channel.
concentration: The concentrations of macromolecule in the titration series.