rfret: Analyze FRET Binding Data

Description Usage Format Source

This dataset was collected on July 26th, 2017. It is a protein-DNA interaction assay, where the DNA is labeled with a fluorophore. This dataset yields a binding constant of 13.28 +/- 0.58 (using the hyperbolic model).

1	fp_binding_data

A data frame with 48 rows and 9 columns. Rows contain two replicates of a titration series, each containing 24 data points. Columns are:

Well Row: The row where the sample corresponding to this record was located into the 384-well microplate.
Well Col: The column where the sample corresponding to this record was located into the 384-well microplate.
Content: A word describing the experiment and replicate this particular data point belongs to (titration.1, titration.2, baseline.1, baseline.2).
Raw Data (parallel): The fluorescence intensities measured in the parallel polarization plane.
Raw Data (perpendicular): The fluorescence intensities measured in the perpendicular polarization plane.
Polarization: The fluorescence polarization values, in mP.
Anisotropy: The fluorescence anisotropy values, in mA.
Intensity: The overall fluorescence intensity values.
concentration: The concentrations of macromolecule in the titration series.