fret_average_replicates: Average technical replicates from a FRET experiment

Description Usage Arguments Value See Also

View source: R/fret_average_replicates.R

Description

This function calculates averages of fluorescence values from an arbitrary number of technical replicates.

Usage

1

Arguments

raw_data

A dataframe containing the raw fluorescence data. It must contain at least 8 columns named:

Experiment

A unique name identifying each experiment.

Type

Either "blank" (no donor) or "titration" (donor present).

Replicate

A number identifying the technical replicate (1, 2, etc.).

Observation

A number identifying each observation in a titration series (corresponds to the plate column numbers, if experiments are set up as rows in a 384-well plate). The number of observations for an experiment and its blanks must match, and a given observation number must associate data points at the same concentration in the titration series.

concentration

The ligand concentration in the titration series.

fret_channel

Fluorescence intensity in the FRET channel.

acceptor_channel

Fluorescence intensity in the acceptor channel.

donor_channel

Fluorescence intensity in the donor channel.

The output of format_data can be used directly as input for this function.

Value

A dataframe containing the reduced dataset after averaging across replicates. It contains all of the above columns except Replicate, because it returns the average values over replicates.

See Also

format_data to prepare datasets for use with fret_average_replicates.


Guilz/rfret documentation built on Oct. 18, 2021, 2:14 p.m.