fp_use_signal: Choose which signal to fit between fluorescence polarization...
In Guilz/rfret: Analyze FRET Binding Data

Description Usage Arguments Value See Also

This function renames the appropriate column in a fluorescence polarization and anisotropy dataset for subsequent fitting by fit_binding_model.

1	fp_use_signal(data, sig = NULL)

data

A dataframe containing the fluorescence polarization and anisotropy data. It must contain at least 7 columns named:

Experiment: A unique name identifying each experiment.
Type: For example, "titration".
Observation: A number identifying each observation in a titration series (corresponds to the plate column numbers, if experiments are set up as rows in a 384-well plate). The number of observations for an experiment and its blanks must match, and a given observation number must associate data points at the same concentration in the titration series.
concentration: The ligand concentration in the titration series.
polarization: Fluorescence polarization.
anisotropy: Fluorescence anisotropy.
intensity: Fluorescence intensity.

The output of fp_average_replicates can be used directly as input for this function.

sig

A character string to identify the signal to use for curve fitting. Possible values are "polarization" or "p" to use fluorescence polarization signal, and "anisotropy" or "a" to use fluorescence anisotropy signal.

A dataframe containing the same dataset with columns appropriately named for fit_binding_model.

format_data and fp_average_replicates.

Guilz/rfret documentation built on Oct. 18, 2021, 2:14 p.m.