ElisaAnalysis: Function to process ELISA data

In econtijoch/Biomass-Workflow: Microbial Density Data Generation and Analysis Tools

Description

Function to process ELISA data

Usage

ElisaAnalysis(plate_reader_file, mapping_file, shiny = FALSE, type = NULL,
  print = FALSE, upper_asymptote = 0.9, growth_rate = 0.5, max_time = 1,
  log_x_max = 3.5, ...)

Arguments

plate_reader_file	the file path of the plate reader file (in .csv or .xls(x) format)
mapping_file	mapping file for plate. Must contain: BarcodeID (unique names for samples), Type ("Standard" or "Experiment" to designate type of sample), SampleMass (to hold standards information to generate standard curve)
shiny	OPTIONAL: necessary for running with shiny app interface since filenames are not the same.
type	OPTIONAL: necessary for running with shiny app, must specify file type
print	OPTIONAL: whether or not to save a copy of the standards curve to the working directory
upper_asymptote	a in the equation: a/(1 + exp(-b * (x-c))) (starting conditions for nls fit)
growth_rate	b in the equation: a/(1 + exp(-b * (x-c))) (starting conditions for nls fit)
max_time	c in the equation: a/(1 + exp(-b * (x-c))) (starting conditions for nls fit)
log_x_max	maximum value of log[Analyte] - used to generate standard curve
...	optional inputs

Value

list containing a table of the standards, and the information for the standard curve

econtijoch/Biomass-Workflow documentation built on May 15, 2019, 7:59 p.m.