Description Usage Arguments Details Value Author(s)
R interface to TrimGalore please see TrimGalore web site at https://github.com/FelixKrueger/TrimGalore/blob/master/Docs/Trim_Galore_User_Guide.md
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 | RunTrimGalore(
file,
paired = TRUE,
quality = 20,
phred33 = TRUE,
phred64 = FALSE,
fastqc = FALSE,
max_length,
error_rate = 0.1,
gzip = TRUE,
length = 20,
maxn,
trimn,
outdir,
report_file = TRUE,
clip_R1,
clip_R2,
three_prime_clip_R1,
three_prime_clip_R2,
retain_unpaired,
length_1 = 35,
length_2 = 35,
trim1,
cores = 4,
remove_intermediate = paired
)
|
file |
a character vector with the file names or the files names to be processed. The fasts should end in .fastq |
quality |
(integer>0) see –quality at TrimGalore web site |
phred33 |
(logical) if TRUE, then –phred33 ON, else –phred33 OFF (see at TrimGalore web site) |
phred64 |
(logical) (see –phred64 at TrimGalore web site) |
max_length |
(integer) (see –max_length at TrimGalore web site) |
error_rate |
(float) default 0.1 (see -e option at TrimGalore web site) |
gzip |
(logical) if TRUE (default) the output files will be zipped (see–gzip and –dont_zip at TrimGalore web site) |
length |
(integer) default 20 (see –length at TrimaGalore web site) |
maxn |
(integer) (see –max_n at TrimGalore web site) |
trimn |
(integer) (see –trim-n at TrimGalore web site) |
outdir |
(character) if missing the output will be stored at the same directory of the (first) input file. (see –output_dir at TrimGalore web site) |
report_file |
(logical) (default TRUE) (see –repot_file at TrimGalore web site) |
clip_R1 |
(integer) (see –clip_R1 at TrimGalore web site) |
clip_R2 |
(integer) (see –clip_R2 at TrimGalore web site) |
three_prime_clip_R1 |
(see –three_prime_clip_R1 at TrimGalore web site) |
three_prime_clip_R2 |
(see –three_prime_clip_R2 at TrimGalore web site) |
retain_unpaired |
(see –retain_unpaired at TrimGalore web site) |
length_1 |
(integer) default 35) (see –length_1 at TrimGalore web site) |
length_2 |
(integer) default 35) (see –length_2 at TrimGalore web site) |
trim1 |
(integer) (see –trim1 at TrimGalore web site) |
cores |
( integer) (default 4) number of cores or threads to use (see –cores at TrimGalore web site) |
remove_intermediate |
remove intermediate trimmed files (only fif paired == TRUE) |
pared |
(TRUE default) if paired == TRUE, the it will look for the second fastq file for each file. The files are expected to be .fastq and identified as XXXX_1.fastq and their corresponding pair XXXX_2.fastq. |
fasqc |
(logical) if TRUE will run fastqc in its default version |
see output files definitions at TrimGalore web site
none
Elmer A. Fernández
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