tests/testthat/test_effector_summary.R

library(effectR)
get_mafft_path <- function(mafft.path = NULL, error = TRUE,
                           verbose = FALSE) {
  # Set default path
  if (is.null(mafft.path)) {
    path <- unname(Sys.which("mafft"))
  } else if (endsWith(mafft.path, "mafft")) {
    path <- mafft.path
  } else if (Sys.info()[['sysname']] %in% "Windows") {
    path <- file.path(mafft.path,"mafft.bat")
  }  else {
    # add on executable to path if not already present
    path <- file.path(mafft.path, "mafft")
  }


  # Check if mafft is installed
  is_installed <- file.exists(path) == T
  if (! is_installed && error) {
    if (is.null(mafft.path)) {
      stop(paste0("MAFFT not found in your computer's search path.",
                  "'\n Please check that MAFFT is installed and in the search path or specify the path to the MAFFT installation using the `mafft.path` option."), call. = FALSE)
    } else {
      stop(paste0("MAFFT not found in the specified path: '", path,
                  "'\n Please check your MAFFT installation."), call. = FALSE)
    }
  }
  return(path)
}

get_hmmer_path <- function(command = "hmmsearch", hmmer.path = NULL, error = TRUE,
                           verbose = FALSE) {
  # Set default path
  if (is.null(hmmer.path)) {
    path <- unname(Sys.which(command))
  } else {
    path <- file.path(hmmer.path, command)
  }
  # Print progress
  if (verbose) {
    message(paste0("Checking if HMMER is installed in the specified path: '", path,"'"))
  }

  # Check if mafft is installed
  is_installed <- system2(path, "-h", stderr = NULL, stdout = NULL) == 0
  if (! is_installed && error) {
    if (is.null(hmmer.path)) {
      stop(paste0("HMMER not found in your computer's search path.",
                  "'\n Please check that HMMER is installed and in the search path or specify the path to the HMMER installation using the `hmm.path` option."), call. = FALSE)
    } else {
      stop(paste0("HMMER not found in the specified path: '", path,
                  "'\n Please check your HMMER installation."), call. = FALSE)
    }
  }

  return(path)
}


test.mafft <- try(get_mafft_path(), silent = T)
test.hmmer <- try(get_hmmer_path(), silent = T)

fasta.file <- system.file("extdata", "test_infestans.fasta", package = 'effectR')
ORF <- seqinr::read.fasta(fasta.file)
REGEX <- regex.search(sequence = ORF, motif = "RxLR")
REGEX.seq <- lapply(REGEX, function (x) paste(unlist(x),collapse = ""))
num.hits <- grep(REGEX.seq, pattern="^\\w{12,60}r\\wlr\\w{6,10}eer", perl = T,ignore.case = T)

context("Testing effector table summary")


if (class(test.mafft) != "try-error" || class(test.hmmer) != "try-error"){
test_that("effectR can read FASTA alignment correctly ", {
  skip_on_cran()
  candidate_rxlr <- hmm.search(original.seq = fasta.file, regex.seq = REGEX)
  summary.list <- effector.summary(candidate.rxlr)
  summary.list.regex <- effector.summary(REGEX)
  expect_equal(class(ORF), "list")
  expect_equal(class(ORF[[1]]), "SeqFastadna")
  expect_equal(length(ORF), 28)
})


test_that("candidate.rxlr returns a list with 3 objects, 17 REGEX, 19 HMM and 19 rows in HMM table ", {
  skip_on_cran()
  candidate.rxlr <- hmm.search(original.seq = fasta.file, regex.seq = REGEX)
  summary.list <- effector.summary(candidate.rxlr)
  summary.list.regex <- effector.summary(REGEX)
  expect_equal(length(candidate.rxlr), 3)
  expect_equal(class(candidate.rxlr), "list")
})

test_that("effector.summary returns a list with 2 objects, 19 candidates and 19 rows in summary table ", {
  skip_on_cran()
  candidate.rxlr <- hmm.search(original.seq = fasta.file, regex.seq = REGEX)
  summary.list <- effector.summary(candidate.rxlr)
  summary.list.regex <- effector.summary(REGEX)
  expect_equal(length(summary.list), 2)
  expect_equal(class(summary.list), "list")
  expect_equal(names(summary.list), c("consensus.sequences","motif.table"))
  expect_equal(length(candidate.rxlr$REGEX), 15)
  expect_equal(length(candidate.rxlr$HMM), 17)
  expect_equal(unique(summary.list$motif.table$MOTIF), c("Complete","Only RxLR motif"))
})

test_that("effector.summary can read REGEX output", {
  skip_on_cran()
  candidate.rxlr <- hmm.search(original.seq = fasta.file, regex.seq = REGEX)
  summary.list <- effector.summary(candidate.rxlr)
  summary.list.regex <- effector.summary(REGEX)
  expect_equal(length(summary.list.regex), 1)
  expect_equal(class(summary.list.regex), "list")
  expect_equal(names(summary.list.regex), "motif.table")
  expect_equal(nrow(summary.list.regex$motif.table), 15)
  expect_equal(unique(summary.list.regex$motif.table$MOTIF), "Complete")
})
}
grunwaldlab/effectR documentation built on Sept. 9, 2023, 1:39 a.m.