Description Usage Arguments Details References
Filters homologous sequences using the BLASTB tool
1 2 3 4 5 6 7 8 | stepBLAST(
x,
word.size = 11,
gapopen = 5,
gapextend = 2,
penalty = -3,
reward = 1
)
|
x |
An object of class |
word.size |
Integer, ... |
gapopen |
Integer, ... |
gapextend |
Integer, ... |
penalty |
Integer, ... |
reward |
Integer, ... |
sseqid | ID of query sequence that gave the lowest E-value |
length | Alignment length |
mismatch | Alignment length |
qstart | Start of alignment in query |
qend | End of alignment in query |
sstart | Start of alignment in subject |
send | End of alignment in subject |
qcovs | Percentage of query coverage per subject |
pident | Percentage of identical matches |
evalue | Expect value |
bitscore | Bit score |
sstrand | Percentage of identical matches |
NCBI BLAST: https://blast.ncbi.nlm.nih.gov/Blast.cgi
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