EBTest: Using EM algorithm to calculate the posterior probabilities...

Description Usage Arguments Details Value Author(s) References See Also Examples

View source: R/EBTest.R

Description

Base on the assumption of NB-Beta Empirical Bayes model, the EM algorithm is used to get the posterior probability of being DE.

Usage

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EBTest(Data, NgVector = NULL, Conditions, sizeFactors, maxround, 
	Pool = F, NumBin = 1000, ApproxVal = 10^-10, Alpha = NULL, 
	Beta = NULL, PInput = NULL, RInput = NULL, 
	PoolLower = .25, PoolUpper = .75, Print = T, Qtrm = 1,QtrmCut=0)

Arguments

Data

A data matrix contains expression values for each transcript (gene or isoform level). In which rows should be transcripts and columns should be samples.

NgVector

A vector indicates the uncertainty group assignment of each isoform. e.g. if we use number of isoforms in the host gene to define the uncertainty groups, suppose the isoform is in a gene with 2 isoforms, Ng of this isoform should be 2. The length of this vector should be the same as the number of rows in Data. If it's gene level data, Ngvector could be left as NULL.

Conditions

A factor indicates the condition which each sample belongs to.

sizeFactors

The normalization factors. It should be a vector with lane specific numbers (the length of the vector should be the same as the number of samples, with the same order as the columns of Data).

maxround

Number of iterations. The default value is 5. Users should always check the convergency by looking at the Alpha and Beta in output. If the hyper-parameter estimations are not converged in 5 iterations, larger number is suggested.

Pool

While working without replicates, user could define the Pool = TRUE in the EBTest function to enable pooling.

NumBin

By defining NumBin = 1000, EBSeq will group the genes with similar means together into 1,000 bins.

PoolLower, PoolUpper

With the assumption that only subset of the genes are DE in the data set, we take genes whose FC are in the PoolLower - PoolUpper quantile of the FC's as the candidate genes (default is 25%-75%).

For each bin, the bin-wise variance estimation is defined as the median of the cross condition variance estimations of the candidate genes within that bin.

We use the cross condition variance estimations for the candidate genes and the bin-wise variance estimations of the host bin for the non-candidate genes.

ApproxVal

The variances of the transcripts with mean < var will be approximated as mean/(1-ApproxVal).

Alpha, Beta, PInput, RInput

If the parameters are known and the user doesn't want to estimate them from the data, user could specify them here.

Print

Whether print the elapsed-time while running the test.

Qtrm, QtrmCut

Transcripts with Qtrm th quantile < = QtrmCut will be removed before testing. The default value is Qtrm = 1 and QtrmCut=0. By default setting, transcripts with all 0's won't be tested.

Details

For each transcript gi within condition, the model assumes: X_gis|mu_gi ~ NB (r_gi0 * l_s, q_gi) q_gi|alpha, beta^N_g ~ Beta (alpha, beta^N_g) In which the l_s is the sizeFactors of samples.

The function will test "H0: q_gi^C1 = q_gi^C2" and "H1: q_gi^C1 != q_gi^C2."

Value

Alpha

Fitted parameter alpha of the prior beta distribution. Rows are the values for each iteration.

Beta

Fitted parameter beta of the prior beta distribution. Rows are the values for each iteration.

P, PFromZ

The bayes estimator of being DE. Rows are the values for each iteration.

Z, PoissonZ

The Posterior Probability of being DE for each transcript(Maybe not in the same order of input).

RList

The fitted values of r for each transcript.

MeanList

The mean of each transcript (across conditions).

VarList

The variance of each transcript (across conditions).

QListi1

The fitted q values of each transcript within condition 1.

QListi2

The fitted q values of each transcript within condition 2.

C1Mean

The mean of each transcript within Condition 1 (adjusted by normalization factors).

C2Mean

The mean of each transcript within Condition 2 (adjusted by normalization factors).

C1EstVar

The estimated variance of each transcript within Condition 1 (adjusted by normalization factors).

C2EstVar

The estimated variance of each transcript within Condition 2 (adjusted by normalization factors).

PoolVar

The variance of each transcript (The pooled value of within condition EstVar).

DataList

A List of data that grouped with Ng.

PPDE

The Posterior Probability of being DE for each transcript (The same order of input).

f0,f1

The likelihood of the prior predictive distribution of being EE or DE (in log scale).

AllZeroIndex

The transcript with expression 0 for all samples (which are not tested).

PPMat

A matrix contains posterior probabilities of being EE (the first column) or DE (the second column). Rows are transcripts. Transcripts with expression 0 for all samples are not shown in this matrix.

PPMatWith0

A matrix contains posterior probabilities of being EE (the first column) or DE (the second column). Rows are transcripts. Transcripts with expression 0 for all samples are shown as PP(EE) = PP(DE) = NA in this matrix. The transcript order is exactly the same as the order of the input data.

ConditionOrder

The condition assignment for C1Mean, C2Mean, etc.

Conditions

The input conditions.

DataNorm

Normalized expression matrix.

Author(s)

Ning Leng

References

Ning Leng, John A. Dawson, James A. Thomson, Victor Ruotti, Anna I. Rissman, Bart M.G. Smits, Jill D. Haag, Michael N. Gould, Ron M. Stewart, and Christina Kendziorski. EBSeq: An empirical Bayes hierarchical model for inference in RNA-seq experiments. Bioinformatics (2013)

See Also

EBMultiTest, PostFC, GetPPMat

Examples

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data(GeneMat)
str(GeneMat)
GeneMat.small = GeneMat[c(1:10,511:550),]
Sizes = MedianNorm(GeneMat.small)
EBOut = EBTest(Data = GeneMat.small,
	Conditions = as.factor(rep(c("C1","C2"), each = 5)),
	sizeFactors = Sizes, maxround = 5)
PP = GetPPMat(EBOut)

lengning/EBSeq documentation built on May 21, 2019, 4:02 a.m.