Make tSNE plots (or PCA, or Monocle; it's customizable)
1 2 3 4 | custom_feature_plot(dge, colour = NULL, subset_id = NULL,
axes = c("tSNE_1", "tSNE_2"), fix_coords = T, show_axes = F,
alpha = 1, cols.use = c("khaki1", "red"), use_rank = F,
overplot_adjust = F)
|
dge |
Seurat object |
colour |
Variable to fetch and plot. Length-1 character. "plain_grey" returns a dark grey plot. |
subset_id |
Vector of subsets to include on the plot. Cells not included will still be included via geom_blank so as to preserve the scale of the plot. |
axes |
Variables to fetch for use on X and Y axes. Defaults to tSNE embedding. |
fix_coords |
Force the plot to be square? Defaults to T, because a rotated or reflected version of the tSNE embedding should convey the exact same relationships. |
alpha |
Transparency. Number between 0 and 1. |
cols.use |
Colorscale. Character vector, hexadecimal or e.g. c("khaki1", "red"). For discrete data fetched for "colour", should be named with the variable's levels. |
use_rank |
Transform expression level to ranks before plotting? |
overplot_adjust |
Bin points within hexagons. Nice for Monocle embeddings. |
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