The output will be a list with a DNAString. The name of the DNAString in the list will be the bin name and will contain information about how many sequences were used to construct the consensus. See construct_consensus.
1 2 3 4 5 6 | process_bin(seqs, classification_technique = "infovar_balance",
classification_params = list(threshold = 1, start_threshold = 0.02,
max_sequences = 100), alignment_technique = "muscle",
alignment_params = list(),
consensus_technique = "Biostrings::consensusString",
consensus_params = list(), remove_gaps = TRUE, strip_uids = FALSE)
|
seqs |
The sequences that were binned together |
classification_technique |
The technique to use to search for mislabelled sequences |
classification_params |
The parameters for the classification technique |
alignment_technique |
The alignment technique to use |
alignment_params |
The alignment parameters to use |
consensus_technique |
The technique to use when generating the consensus sequence |
consensus_params |
The parameters for the consensus generator |
remove_gaps |
If set to TRUE (the default, then gaps will be removed from the consensus sequences) |
strip_uids |
Remove the unique identifiers from the sequence. It is not intelligent. The names will be split on '_' and the first and last pieces will be kept. |
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