FlowStandards: An S4 class to represent internal standard details for...

In plantarum/flowPloidy: Analyze flow cytometer data to determine sample ploidy

An S4 class to represent internal standard details for FlowHist objects

Description

The sizes slot is set in FlowHist or batchFlowHist. The other values are updates through interaction with the browseFlowHist GUI.

Usage

stdSizes(std)

stdSelected(std)

stdPeak(std)

Arguments

std	a FlowStandards object

Value

stdSizes, stdSelected and stdPeak return the corresponding slot values

Slots

sizes

numeric, the size (in pg) of the internal size standard. Can be a vector of multiple values, if the sample is part of a set that included different standards for different samples.

selected

numeric, the size (in pg) of the internal size standard actually used for this sample. Must be one of the values in the sizes slot.

peak

character, "A" or "B", indicating which of the histogram peaks is the size standard.

Examples

library(flowPloidyData) 
fh1 <- FlowHist(file = flowPloidyFiles()[1], channel = "FL3.INT.LIN",
                standards = c(1.96, 5.43))
fhStandards(fh1)  ## display standards included in this object
stdSizes(fhStandards(fh1))  ## list standard sizes

plantarum/flowPloidy documentation built on March 25, 2023, 1:37 a.m.