updateFlowHist: updateFlowHist

In plantarum/flowPloidy: Analyze flow cytometer data to determine sample ploidy

updateFlowHist

Description

Update, and optionally re-analyze, a FlowHist object

Usage

updateFlowHist(
  fh,
  linearity = NULL,
  debris = NULL,
  samples = NULL,
  analyze = TRUE
)

Arguments

fh	a FlowHist object
linearity	character, either "variable", the default, or "fixed". If "fixed", linearity is fixed at 2; if "variable", linearity is fit as a model parameter.
debris	character, either "SC", the default, or "MC", to set the debris model component to the Single-Cut or Multi-Cut models.
samples	integer, the number of samples in the data
analyze	logical, if TRUE the updated model will be analyzed immediately

Details

Allows users to switch the debris model from Single-Cut to Multi-Cut (or vice-versa), or to toggle linearity between fixed and variable.

Value

a FlowHist object with the modified values of linearity and/or debris, and, if analyze was TRUE, a new NLS fitting

Author(s)

Tyler Smith

Examples

## defaults to 256 bins:
library(flowPloidyData) 
fh1 <- FlowHist(file = flowPloidyFiles()[1], channel = "FL3.INT.LIN")
## default is Single-Cut, change that to Multi-Cut:
fh1mc <- updateFlowHist(fh1, debris = "MC")
plot(fh1)

plantarum/flowPloidy documentation built on March 25, 2023, 1:37 a.m.