fhStart: Calculate the where to start analysis for a 'FlowHist'...
In plantarum/flowPloidy: Analyze flow cytometer data to determine sample ploidy
fhStart R Documentation
Calculate the where to start analysis for a FlowHist
histogram
Description
We exclude the first five bins at the outset (as part of the function
setBins. For some flow cytometers, these values contain
very high spikes that are an artifact of compensation, and are not
useful data.
Usage
fhStart(intensity)
Arguments
intensity
numeric, the fluorescence intensity channel bins
Details
After that, we call fhStart to skip to the highest value
in the first 10 non-zero bins, and ignore everything below that. The
motivation here is the same - to get out beyond the noisy bins and into
the actual data we're trying to fit.
Value
an integer, the index of the first intensity element to include
in the actual model fitting. That is, everything from startBin
to the end of intensity gets fit in the model, everything below
startBin is ignored.
Author(s)
Tyler Smith
plantarum/flowPloidy documentation built on March 25, 2023, 1:37 a.m.
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| fhStart | R Documentation |
Calculate the where to start analysis for a FlowHist
histogram
Description
We exclude the first five bins at the outset (as part of the function
setBins. For some flow cytometers, these values contain
very high spikes that are an artifact of compensation, and are not
useful data.
Usage
fhStart(intensity)
Arguments
intensity |
numeric, the fluorescence intensity channel bins |
Details
After that, we call fhStart to skip to the highest value
in the first 10 non-zero bins, and ignore everything below that. The
motivation here is the same - to get out beyond the noisy bins and into
the actual data we're trying to fit.
Value
an integer, the index of the first intensity element to include
in the actual model fitting. That is, everything from startBin
to the end of intensity gets fit in the model, everything below
startBin is ignored.
Author(s)
Tyler Smith
R Package Documentation
Browse R Packages
We want your feedback!
Note that we can't provide technical support on individual packages. You should contact the package authors for that.
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