setBins: setBins

In plantarum/flowPloidy: Analyze flow cytometer data to determine sample ploidy

setBins

Description

(Re-)set the bins for a FlowHist object

Usage

setBins(fh, bins = 256)

Arguments

fh	a FlowHist object
bins	integer, the number of bins to use in aggregating FCS data

Details

This function sets (or resets) the number of bins to use in aggregating FCS data into a histogram, and generates the corresponding data matrix. Not exported for general use.

The histData matrix also contains the columns corresponding to the raw data used in calculating the single-cut and multiple-cut debris components, as well as the doublet, triplet, and quadruplet aggregate values. (i.e., SCvals, MCvals, DBvals, TRvals, and QDvals).

setBins includes a call to resetFlowHist, so all the model components that depend on the bins are updated in the process (as you want!).

Value

a FlowHist object, with the bins slot set to bins, and the corresonding binned data stored in a matrix in the histData slot. Any previous analysis slots are removed: peaks, comps, model, init, nls, counts, CV, RCS.

Author(s)

Tyler Smith

Examples

## defaults to 256 bins:
library(flowPloidyData) 
fh1 <- FlowHist(file = flowPloidyFiles()[1], channel = "FL3.INT.LIN")
plot(fh1)
## reset them to 512 bins:
fh1 <- setBins(fh1, 512)
plot(fh1)

plantarum/flowPloidy documentation built on March 25, 2023, 1:37 a.m.