Description Usage Arguments Details Value Warning Author(s) References See Also Examples
Principal Response Curves (PRC) are a special case of
Redundancy Analysis (rda
) for multivariate responses in
repeated observation design. They were originally suggested for
ecological communities. They should be easier to interpret than
traditional constrained ordination. They can also be used to study how
the effects of a factor A
depend on the levels of a factor
B
, that is A + A:B
, in a multivariate response
experiment.
1 2 3 4 5 6 7 8 | prc(response, treatment, time, ...)
## S3 method for class 'prc'
summary(object, axis = 1, scaling = "symmetric",
digits = 4, correlation = FALSE, ...)
## S3 method for class 'prc'
plot(x, species = TRUE, select, scaling = "symmetric",
axis = 1, correlation = FALSE, type = "l", xlab, ylab, ylim,
lty = 1:5, col = 1:6, pch, legpos, cex = 0.8, ...)
|
response |
Multivariate response data. Typically these are community (species) data. If the data are counts, they probably should be log transformed prior to the analysis. |
treatment |
A factor for treatments. |
time |
An unordered factor defining the observations times in the repeated design. |
object, x |
An |
axis |
Axis shown (only one axis can be selected). |
scaling |
Scaling of species scores, identical to the
The type of scores can also be specified as one of |
digits |
Number of significant digits displayed. |
correlation |
logical; if |
species |
Display species scores. |
select |
Vector to select displayed species. This can be a vector
of indices or a logical vector which is |
type |
Type of plot: |
xlab, ylab |
Text to replace default axis labels. |
ylim |
Limits for the vertical axis. |
lty, col, pch |
Line type, colour and plotting characters (defaults supplied). |
legpos |
The position of the |
cex |
Character expansion for symbols and species labels. |
... |
Other parameters passed to functions. |
PRC is a special case of rda
with a single
factor for treatment
and a single factor for time
points
in repeated observations. In vegan, the corresponding
rda
model is defined as rda(response ~ treatment *
time + Condition(time))
. Since the time
appears twice in the
model formula, its main effects will be aliased, and only the main
effect of treatment and interaction terms are available, and will be
used in PRC. Instead of usual multivariate ordination diagrams, PRC
uses canonical (regression) coefficients and species scores for a
single axis. All that the current functions do is to provide a special
summary
and plot
methods that display the
rda
results in the PRC fashion. The current version only
works with default contrasts (contr.treatment
) in which
the coefficients are contrasts against the first level, and the levels
must be arranged so that the first level is the control (or a
baseline). If necessary, you must change the baseline level with
function relevel
.
Function summary
prints the species scores and the
coefficients. Function plot
plots coefficients against
time
using matplot
, and has similar defaults.
The graph (and PRC) is meaningful only if the first treatment
level is the control, as the results are contrasts to the first level
when unordered factors are used. The plot also displays species scores
on the right vertical axis using function
linestack
. Typically the number of species is so high
that not all can be displayed with the default settings, but users can
reduce character size or padding (air
) in
linestack
, or select
only a subset of the
species. A legend will be displayed unless suppressed with
legpos = NA
, and the functions tries to guess where to put the
legend if legpos
is not supplied.
The function is a special case of rda
and returns its
result object (see cca.object
). However, a special
summary
and plot
methods display returns differently
than in rda
.
The first level of treatment
must be the
control: use function relevel
to guarantee the correct
reference level. The current version will ignore user setting of
contrasts
and always use treatment contrasts
(contr.treatment
). The time
must be an unordered
factor.
Jari Oksanen and Cajo ter Braak
van den Brink, P.J. & ter Braak, C.J.F. (1999). Principal response curves: Analysis of time-dependent multivariate responses of biological community to stress. Environmental Toxicology and Chemistry, 18, 138–148.
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 | ## Chlorpyrifos experiment and experimental design: Pesticide
## treatment in ditches (replicated) and followed over from 4 weeks
## before to 24 weeks after exposure
data(pyrifos)
week <- gl(11, 12, labels=c(-4, -1, 0.1, 1, 2, 4, 8, 12, 15, 19, 24))
dose <- factor(rep(c(0.1, 0, 0, 0.9, 0, 44, 6, 0.1, 44, 0.9, 0, 6), 11))
ditch <- gl(12, 1, length=132)
# PRC
mod <- prc(pyrifos, dose, week)
mod # RDA
summary(mod) # PRC
logabu <- colSums(pyrifos)
plot(mod, select = logabu > 100)
## Ditches are randomized, we have a time series, and are only
## interested in the first axis
ctrl <- how(plots = Plots(strata = ditch,type = "free"),
within = Within(type = "series"), nperm = 99)
anova(mod, permutations = ctrl, first=TRUE)
|
Loading required package: permute
Loading required package: lattice
This is vegan 2.4-3
Call: prc(response = pyrifos, treatment = dose, time = week)
Inertia Proportion Rank
Total 288.9920 1.0000
Conditional 63.3493 0.2192 10
Constrained 96.6837 0.3346 44
Unconstrained 128.9589 0.4462 77
Inertia is variance
Eigenvalues for constrained axes:
RDA1 RDA2 RDA3 RDA4 RDA5 RDA6 RDA7 RDA8 RDA9 RDA10 RDA11
25.282 8.297 6.044 4.766 4.148 3.857 3.587 3.334 3.087 2.551 2.466
RDA12 RDA13 RDA14 RDA15 RDA16 RDA17 RDA18 RDA19 RDA20 RDA21 RDA22
2.209 2.129 1.941 1.799 1.622 1.579 1.440 1.398 1.284 1.211 1.133
RDA23 RDA24 RDA25 RDA26 RDA27 RDA28 RDA29 RDA30 RDA31 RDA32 RDA33
1.001 0.923 0.862 0.788 0.750 0.712 0.685 0.611 0.584 0.537 0.516
RDA34 RDA35 RDA36 RDA37 RDA38 RDA39 RDA40 RDA41 RDA42 RDA43 RDA44
0.442 0.417 0.404 0.368 0.340 0.339 0.306 0.279 0.271 0.205 0.179
Eigenvalues for unconstrained axes:
PC1 PC2 PC3 PC4 PC5 PC6 PC7 PC8
17.156 9.189 7.585 6.064 5.730 4.843 4.518 4.105
(Showed only 8 of all 77 unconstrained eigenvalues)
Call:
prc(response = pyrifos, treatment = dose, time = week)
Species scores:
Simve Daplo Cerpu Alogu Aloco Alore Aloaf Copsp
-1.461934 -0.796510 -0.295171 -0.152301 -0.096273 -0.171335 -0.231967 -0.635966
Ostsp Slyla Acrha Aloex Chysp Alona Plead Oxyte
-1.257492 0.302872 -0.057396 -0.124049 -0.051689 -0.034638 -0.075268 -0.013815
Grate Copdi NauLa CilHa Strvi amosp Ascmo Synsp
-0.052667 -0.777088 -2.636100 -0.486881 -1.669474 0.738371 -0.037926 0.014409
Squro Squmu Polar Kerqu Anufi Mytve Mytvi Mytmu
-0.143790 0.246185 -0.251255 -0.269397 -0.235362 -0.040448 -0.049452 -0.057589
Lepsp Leppa Colob Colbi Colun Lecsp Lecqu Lecco
-0.542922 -0.046123 0.393234 0.075905 0.450495 -0.257170 0.048327 0.158007
Leclu Lecfl Tripo Cepsp Monlo Monae Scalo Trilo
-0.027077 0.221912 -0.117056 0.440303 0.287108 0.048918 0.041981 0.021257
Tripo.1 Tricy Trisp Tepat Rotne Notla Filsp Lopox
-0.134025 -0.182409 -0.042651 -0.004198 -0.078168 0.062163 0.091561 0.016854
hydrspec bothrosp olchaeta erpoocto glsicomp alglhete hebdstag sphidae
0.026485 -0.216815 0.633674 0.490029 0.078527 0.039728 -0.490678 -0.796015
ansuvote armicris bathcont binitent gyraalbu hippcomp lymnstag lymnaes7
-0.076512 -0.913681 -0.039855 1.060788 -0.017975 -0.219974 0.143403 -0.073502
physfont plbacorn popyanti radiovat radipere valvcris valvpisc hycarina
0.014349 -0.046098 -0.691904 0.010777 0.340163 -0.005753 0.145523 -0.567803
gammpule aselaqua proameri collembo caenhora caenluct caenrobu cloedipt
-0.830166 -0.858606 -0.063401 -0.016264 -3.136867 -1.292300 -0.068624 -2.574625
cloesimi aeshniae libellae conagrae corident coripanz coripunc cymabons
-0.675580 -0.115677 0.044524 -0.886795 -0.007484 -0.065501 0.096124 -0.025213
hesplinn hespsahl notoglau notomacu notoobli notoviri pacoconc pleaminu
0.037779 -0.018078 -0.301948 -0.027226 -0.044790 -0.117398 -0.009087 -0.038705
sigadist sigafall sigastri sigarasp colyfusc donacis6 gyrimari haliconf
-0.041594 0.009987 -0.032743 0.150817 -0.019847 0.042751 -0.005753 -0.243054
haliflav haligruf haliobli herubrev hya_herm hyglpusi hyhyovat hypoplan
-0.024222 -0.244814 -0.071501 0.069877 0.175327 0.006404 -0.013159 -0.008145
hyporusp hytuinae hytuvers laphminu noteclav rhantusp sialluta ablalong
-0.126197 -1.259664 -0.963901 -0.344204 -0.004303 -0.036774 -0.603320 -0.008145
ablaphmo cltanerv malopisp mopetenu prdiussp pstavari chironsp crchirsp
-1.627590 -0.041132 -0.025919 -0.004740 -0.301790 -0.045054 -1.027839 -0.009087
crclglat ditendsp mitegchl pachgarc pachgvit popegnub popedisp acriluce
-0.015746 -0.045402 -0.125429 0.006628 0.016265 -0.121971 0.037879 0.004324
chclpige conescut cricotsp liesspec psclbarb psclgsli psclobvi psclplat
0.004756 -0.446524 -0.066095 -0.058403 0.015576 -0.327165 0.197081 0.028310
psclpsil pscladsp cladotsp laa_spec patanysp tatarssp zaa_spec anopmacu
-0.003991 -0.003086 -0.293623 -0.018548 -0.079841 -0.364072 -0.027165 0.089046
cepogoae chaoobsc cucidae4 tabanusp agdasphr athrater cyrncren holodubi
-1.389767 -1.328261 -0.018078 0.006309 -0.147828 -0.036774 -0.038705 -0.051532
holopici leceriae lilurhom monaangu mystazur mystloni oecefurv oecelacu
-0.332631 -0.162413 -0.004929 -0.350402 -0.018078 -1.630725 -0.291847 -0.140893
triabico paponysp
-0.048357 -0.053182
Coefficients for dose + week:dose interaction
which are contrasts to dose 0
rows are dose, columns are week
-4 -1 0.1 1 2 4 8 12 15 19
0.1 0.1327 0.2527 0.1875 0.07479 0.3863 0.2509 0.1485 0.2824 0.2064 0.3977
0.9 0.1490 0.3558 0.3560 0.87706 0.9151 0.7918 0.2488 0.6523 0.4428 0.3229
6 0.3055 0.2266 0.8346 2.12626 1.9923 2.1165 1.0317 0.8638 0.5660 0.6054
44 0.2570 0.3601 1.3438 2.31842 2.3862 2.6894 2.3745 1.8536 1.4377 1.0607
24
0.1 0.1441
0.9 0.2839
6 0.3352
44 0.5704
Permutation test for rda under reduced model
Plots: ditch, plot permutation: free
Permutation: series
Number of permutations: 99
Model: prc(response = pyrifos, treatment = dose, time = week)
Df Variance F Pr(>F)
RDA1 1 25.282 15.096 0.01 **
Residual 77 128.959
---
Signif. codes: 0 '***' 0.001 '**' 0.01 '*' 0.05 '.' 0.1 ' ' 1
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