Description Usage Arguments Value
View source: R/internal_fluo_est.R
It estimates the spot or capture site location by BF image modelling.
1 | extractBFArea(cc, img, area, BFarea)
|
cc |
Data matrix. An estimated spot center. |
img |
Data matrix. The matrix of image data from a channel. |
area |
Data matrix. The bright (spot) coordinates around the spot center. |
BFarea |
Integer. Defines a rectangular pseudo-spot size whose fluorescence will be estimated. |
The area identified by BF image modelling
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