extractBFarea

Description

It estimates the spot or capture site location by BF image modelling.

Usage

1
extractBFArea(cc, img, area, BFarea)

Arguments

cc

Data matrix. An estimated spot center.

img

Data matrix. The matrix of image data from a channel.

area

Data matrix. The bright (spot) coordinates around the spot center.

BFarea

Integer. Defines a rectangular pseudo-spot size whose fluorescence will be estimated.

Value

The area identified by BF image modelling

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