Description Usage Arguments Value

View source: R/internal_fluo_NBE.R

It performs the run effect correction and normalization of the raw fluorescence signals.

1 2 | ```
adjustFluo(data, transformation, BGmethod, maxMix, reference, prior.pi,
flex.reps, flexmethod, image.type, savePlot, seed, dateIndex)
``` |

`data` |
List. A list with the fluorescence signal information of both channels. |

`BGmethod` |
Character string. The type of image background correction to be performed. One of "normexp" or "subtract". |

`maxMix` |
Integer. The maximum number of components to fit into the mixture of regressions model. If maxMix=1 or if the the optimal number of the estimated components is 1, the model reduces to the classical 2-way ANOVA. |

`reference` |
Numeric vector. Specifies the runs to be used as baseline (iteratively). |

`prior.pi` |
Float. The prior probability to accept a component. |

`flex.reps` |
Integer. The iterations of the Expectation-Maximization algorithm to estimate the flexmix model. |

`flexmethod` |
Character string. A method to estimate the optimal number of flexmix components. One of "BIC", "AIC", "ICL". |

`image.type` |
Character string. A triplet of IDs to characterize the type of images under study. |

`savePlot` |
Character string. The directory to store the plots or an option to print them on the screen. |

`seed` |
Integer. An optional seed number for the Random Number Generator. |

`dateIndex` |
Character string. a date index to be used in saving the output files. |

`transform` |
Character string. The type of transformation to be performed. One of "bc" (Box-Cox), "log", "log10" or "asinh". |

A list with the fluorescence signals, mixture components and flexmix model estimates

CONFESS documentation built on Nov. 17, 2017, 8:55 a.m.

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