# Calculate the posterior fold change for each transcript across conditions

### Description

'PostFC' calculates the posterior fold change for each transcript across conditions.

### Usage

1 | ```
PostFC(EBoutput, SmallNum = 0.01)
``` |

### Arguments

`EBoutput` |
The ourput from function EBTest. |

`SmallNum` |
A small number will be added for each transcript in each condition to avoid Inf and NA. Default is 0.01. |

### Value

Provide both FC and posterior FC across two conditions. FC is calculated as (MeanC1+SmallNum)/(MeanC2+SmallNum). And Posterior FC is calculated as:

# Post alpha P_a_C1 = alpha + r_C1 * n_C1

# Post beta P_b_C1 = beta + Mean_C1 * n_C1

# P_q_C1 = P_a_C1 / (P_a_C1 + P_b_C1)

# Post FC = ((1-P_q_C1)/P_q_c1) / ( (1-P_q_c2)/P_q_c2)

`PostFC` |
The posterior FC across two conditions. |

`RealFC` |
The FC across two conditions (adjusted by the normalization factors). |

`Direction` |
The diretion of FC calculation. |

### Author(s)

Ning Leng

### References

Ning Leng, John A. Dawson, James A. Thomson, Victor Ruotti, Anna I. Rissman, Bart M.G. Smits, Jill D. Haag, Michael N. Gould, Ron M. Stewart, and Christina Kendziorski. EBSeq: An empirical Bayes hierarchical model for inference in RNA-seq experiments. Bioinformatics (2013)

### See Also

EBTest, GetMultiFC

### Examples

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