alignSequences: Align sequences via clustalo
In LowMACA: LowMACA - Low frequency Mutation Analysis via Consensus Alignment

Description Usage Arguments Details Value Warning Author(s) References See Also Examples

Align sequences for an object of class LowMACA

1 2	alignSequences(object, clustalo_filename=NULL , mail=NULL , perlCommand="perl", use_hmm=FALSE, datum=FALSE)

`object`	an object of class LowMACA containing at least 2 sequences.
`clustalo_filename`	a character string that contains the file name where clustal omega alignment file will be stored. In case it's NULL no file will be written. Default=NULL
`mail`	a character string indicating the email address where error report should be sent in web mode
`perlCommand`	a character string containing the path to Perl executable. if missing, "perl" will be used as default
`use_hmm`	When analysing Pfam sequences, it is possible to use the Hidden Markov Model (HMM) of the specific Pfam to align the sequences. Default is FALSE.
`datum`	When analysing Pfam sequences, use all the genes that belong to the Pfam to generate the alignment. This creates a unique mapping between individual residues and consensus sequence, disregarding the set of sequences that are selected for the analysis. Default is FALSE.

This method launches a system call to clustalo aligner and optionally creates a fasta file in clustal format. A warning is returned if at least one sequence has a pairwise similarity below 20% to any other sequence. If only one sequence is passed to alignSequences, the alignment will be skipped, but no warning will be raised. If mail is not NULL, a local installation of clustal omega is no longer required and the alignment is performed using clustal omega EBI web service. A limit of 2000 sequences is set in this case and perl must be installed in the system

The method returns an object of class LowMACA updating the slot alignment. See lmAlignment

When a sequence has a similarity below 20%, a warning is raised. In order to produce strong results in terms of conservation of multiple mutations, consider to remove that sequence from the analysis. The alignment will obviously change.

Stefano de Pretis, Giorgio Melloni

Trident Score Clustal Omega Clustal Omega Web Service

getMutations , mapMutations , setup

#Create an object of class LowMACA for RAS domain family
lm <- newLowMACA(pfam="PF00071" , genes=c("KRAS" , "NRAS" , "HRAS"))
#Align sequences using local installation of clustalo
lm <- alignSequences(lm)
#Web service clustalomega aligner
lm <- alignSequences(lm , mail="lowmaca@gmail.com")
#Use HMM to align
lm <- alignSequences(lm , use_hmm=TRUE)
#Use "datum"
lm <- alignSequences(lm , datum=TRUE)