inst/testScripts/system/chipTypes/GenomeWideSNP_6/21.doASCRMAv2,ArrayExplorer.R

library("aroma.affymetrix")
verbose <- Arguments$getVerbose(-4, timestamp=TRUE)


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# Setup
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dataSet <- "GSE13372,testset"
chipType <- "GenomeWideSNP_6,Full"

csR <- AffymetrixCelSet$byName(dataSet, chipType=chipType, verbose=verbose)
print(csR)


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# Spatial intensity plots
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ae <- ArrayExplorer(csR)
setColorMaps(ae, "sqrt,yellow")
print(ae)

process(ae, verbose=verbose)



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# Spatial probe log-ratio plots
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cfR <- getAverageFile(csR, verbose=verbose)
reporter <- SpatialReporter(csR, reference=cfR)
addColorMap(reporter, "log2center,rainbow")
process(reporter, zrange=c(-2,2), verbose=verbose)

ylab <- expression(log[2](y/y[R]))
for (ii in seq_along(csR)) {
  df <- csR[[ii]]

  toPNG(getFullName(df), tags="spatial,rowMedians", width=300, aspectRatio=8/3, {
    plotMargins(reporter, array=ii, ylim=c(-1,1)*0.2, ylab=ylab, margins="rows", rotate=90)
  })

  toPNG(getFullName(df), tags="spatial,colMedians", width=800, aspectRatio=3/8, {
    plotMargins(reporter, array=ii, ylim=c(-1,1)*0.2, ylab=ylab, margins="columns", rotate=0)
  })
} # for (ii ...)


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# Spatial residual plots test
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res <- doASCRMAv2(csR, drop=FALSE, verbose=verbose)
plm <- res$plm
print(plm)

# Calculate PLM residuals
rs <- calculateResidualSet(plm, verbose=verbose)
print(rs)

ae <- ArrayExplorer(rs)
setColorMaps(ae, c("log2,log2neg,rainbow", "log2,log2pos,rainbow"))
print(ae)

process(ae, interleaved="auto", verbose=verbose)

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aroma.affymetrix documentation built on July 18, 2022, 5:07 p.m.