HexNAc - Analysis of N-HexNAc glycopeptides by LC-MS/MS, using HCD and ETD fragmentation techniques

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Description

HexNAc is intented for demonstration of the PTM_MarkerFinder methode descriped in [PROTEOMICS pmic.201300036]. It contains the mass pattern (m/z) of c(126.05495, 138.05495, 144.06552, 168.06552, 186.07608, 204.08665).

A sample containing enriched glycopeptides from yeast was analysed by LC-MS/MS on a LTQ-Orbitrap Velos instrument using sequential HCD and ETD fragmentation techniques. The dataset contains only 11 tandem mass spectra extracted from this experiment. Three pairs of HCD/ETD spectra correspond to peptides carrying N-HexNAc modification identified with high confidence (6 spectra in total). One pair of HCD/ETD spectra corresponds to peptides carrying N-HexNAc modification, identified with very low confidence (2 spectra in total). The remaining 3 spectra are from unmodified peptides.

Format

A data set consists of eleven tandem mass spectra.

Author(s)

Paolo Nanni, Christian Panse, 2013

Examples

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    data(HexNAc)
    HexNAc[[1]]

    plot(HexNAc[[1]]$mZ, HexNAc[[1]]$intensity, type='h')

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