HexNAc - Analysis of N-HexNAc glycopeptides by LC-MS/MS, using HCD and ETD fragmentation techniques
HexNAc is intented for demonstration of the
descriped in [PROTEOMICS pmic.201300036].
It contains the mass pattern (m/z) of
c(126.05495, 138.05495, 144.06552, 168.06552, 186.07608, 204.08665).
A sample containing enriched glycopeptides from yeast was analysed by LC-MS/MS on a LTQ-Orbitrap Velos instrument using sequential HCD and ETD fragmentation techniques. The dataset contains only 11 tandem mass spectra extracted from this experiment. Three pairs of HCD/ETD spectra correspond to peptides carrying N-HexNAc modification identified with high confidence (6 spectra in total). One pair of HCD/ETD spectra corresponds to peptides carrying N-HexNAc modification, identified with very low confidence (2 spectra in total). The remaining 3 spectra are from unmodified peptides.
A data set consists of eleven tandem mass spectra.
Paolo Nanni, Christian Panse, 2013
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