pressureProfile: NanoLC pressure profile
In protViz: Visualizing and Analyzing Mass Spectrometry Related Data in Proteomics

pressureProfile

R Documentation

NanoLC pressure profile

Description

pressureProfile is a data frame with a variable number of cases (rows) and two variables (columns) named time(min), Pc(psi)

Details

The pressureProfile data set gives the flow profile in nL/min and the pressure profile in psi as a function of time, respectively, for 24 consecutive injections on an Eksigent NanoLC-Ultra 1D plus system.

The dataset consists of 24 HPLC pressure profiles of 140 min. Data was acquired with a Eksigent NanoLC-Ultra 1D plus system. Samples were separated with a 100 1E-6m ID. column of 150 mm lenght packed with C-18 AQ 200A beads (Dr. Maisch GmbH). The column was heated to a temperature of 50 grad C during the entire run. Acquisition queue was as follows:

- A block of three (3) samples with a gradient length of 140 min was allways followed a autocalibration run with a gradient length of 25 min.

- A total of seven (7) such blocks was acquired to cover the entire dataset.

Author(s)

Christian Trachsel 2012

Examples


data(pressureProfile)
ppp(pressureProfile[pressureProfile$filename=="F01",])

par(mfrow=c(1,1))
pps.data<-pps(pressureProfile, time=seq(1,140,by=5))
boxplot(Pc~time, data=pps.data, 
    xlab='time [in minutes]', ylab='Pc(psi)')

library(lattice)
pps.data<-pps(pressureProfile, time=seq(25,40,by=5))
xyplot(Pc ~ as.factor(file) | paste("time =", as.character(time), "minutes"),
    panel = function(x, y){
        m<-sum(y)/length(y)
        m5<-(max(y)-min(y))*0.05
        panel.abline(h=c(m-m5,m,m+m5), col=rep("#ffcccc",3),lwd=c(2,4,2))
        panel.grid(h=-1, v=0)
        panel.xyplot(x, y)
    },
    ylab='Pc [psi]',
    layout=c(1,4),
    sub='The three read lines indicate avg plus min 5%.',
    scales = list(x = list(rot = 45)),
    data=pps.data)

protViz documentation built on May 29, 2024, 8:21 a.m.