The purpose of
msms is for the demonstration of the peptide
identification methods, e.g.,
msms contains two tandem mass spectra meassured on a linear ion
trap coupled to an Orbitrap mass analyzer
(Thermo Scientific, Bremen, Germany).
The protein sample originates from 10 mouse brain homogenate, prepared in sterile PBS containing protease inhibitors (Complete; Riche, Switzerland) by repeated extrusion through syringe needles of successive smaller size. To remove gross cellular debris the homogenate was centrifuged for 10 min at 2400 rpm at 4 degrees centigrade.
The data were acquired withing the Sixth Framework Programm (FP6-37457). The project was called SysProt.
Dorothea Rutishauser, Jonas Grossmann, Christian Panse 2008
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