edgeOF | R Documentation |
This function will flag objects on the edge of wells that are often more difficult to segment properly with CellProfiler.
edgeOF(data, radius = 825, center_x = 1024, center_y = 1024)
data |
A data frame output from the |
radius |
Radius in pixels away from image center with even illumination. The default value is set to 825, which is standard for images taken with the imageXpress nano 2X objective. |
center_x |
center x position of image in pixels. Default is 1024. |
center_y |
center y position of image in pixels. Default is 1024. |
A single data frame with edge_ObjectFlag
and well_edge_flag_radius
variables added. The edge_ObjectFlag
variable is coded as "edge"
for objects with centroids outside
the radius provided, all other objects are coded as NA_character
.
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