edgeOF: edgeOF

In AndersenLab/easyXpress: A Package to Read, Process, and Analyze Worm Data Generated from CellProfiler’s WormToolbox

edgeOF

Description

This function will flag objects on the edge of wells that are often more difficult to segment properly with CellProfiler.

Usage

edgeOF(data, radius = 825, center_x = 1024, center_y = 1024)

Arguments

data	A data frame output from the modelSelection function.
radius	Radius in pixels away from image center with even illumination. The default value is set to 825, which is standard for images taken with the imageXpress nano 2X objective.
center_x	center x position of image in pixels. Default is 1024.
center_y	center y position of image in pixels. Default is 1024.

Value

A single data frame with edge_ObjectFlag and well_edge_flag_radius variables added. The edge_ObjectFlag variable is coded as "edge" for objects with centroids outside the radius provided, all other objects are coded as NA_character.

AndersenLab/easyXpress documentation built on Dec. 6, 2024, 4:04 a.m.