R/PlotAllDynamicRange.R

Defines functions PlotAllDynamicRange

Documented in PlotAllDynamicRange

#' Plots the dynamic range for all samples
#'
#' @param MQCombined Object list containing all the files from the MaxQuant
#' output. It is the result from using \code{make_MQCombined}.
#' @param show_shade Creates a shade showing where the \code{percent_proteins}
#' are.
#'  Default is TRUE.
#' @param percent_proteins Determines the percentage for the show_shade
#' parameter.
#'  Default is 0.90 (90\% of the proteins).
#'
#' @return Returns one plot for each sample, being the dynamic range.
#' @export
#'
#' @examples
#' MQPathCombined <- system.file("extdata/combined/", package = "MQmetrics")
#' MQCombined <- make_MQCombined(MQPathCombined)
#' PlotAllDynamicRange(MQCombined)
PlotAllDynamicRange <- function(MQCombined,
                                show_shade = TRUE,
                                percent_proteins = 0.90) {

    proteinGroups <- MQCombined$proteinGroups.txt



    rank_groups <- proteinGroups %>%
        select(contains("Intensity ")) %>%
        select(-starts_with("LFQ"))

    rank_groups <- log10(rank_groups)

    colnames(rank_groups) <- gsub("Intensity", "", colnames(rank_groups))

    pl <- vector("list", length = ncol(rank_groups))


    ## columns and rows depending of number of samples

    if (ncol(rank_groups) <= 4) {
        columns_grid <- 1
        rows_grid <- 4
    }

    if (ncol(rank_groups) > 4) {
        columns_grid <- 2
        rows_grid <- 4
    }

    for (i in seq_len(ncol(rank_groups))) {
        temp <- data.frame(rank_groups[, i])

        colnames(temp) <- colnames(rank_groups)[i]

        assign(colnames(rank_groups)[i], temp)

        temp <- temp[order(temp[, 1], decreasing = TRUE), ]

        temp <- temp[!grepl("^-Inf$", temp)]


        vec_temp <- seq_len(length(temp))

        temp_data <- data.frame(vec_temp, temp)

        temp_plot <- ggplot(temp_data, aes(x = vec_temp, y = temp)) +
            ggtitle(colnames(rank_groups)[i]) +
            theme_bw() +
            theme(panel.grid.major = element_blank(),
                  panel.grid.minor = element_blank())+
            ylab(expression("Log"[10] * "(Intensity)")) +
            xlab("Protein Abundance Rank")

        if (show_shade == TRUE) {
            limits <- (1 - percent_proteins) / 2

            limits_row <- round(nrow(temp_data) * 0.05)

            upper_y <- temp_data$temp[limits_row]

            bottom_y <- temp_data$temp[nrow(temp_data) - limits_row]

            orders_abundance_temp <- paste(
                round(upper_y - bottom_y, digits = 1),
                "orders  of abundance"
                )

            temp_plot <- temp_plot +
                annotate("rect",
                        xmin = limits_row,
                        xmax = nrow(temp_data) - limits_row,
                        ymin = bottom_y,
                        ymax = upper_y,
                        alpha = 0.3,
                        fill = '#5B84B1FF'
                        ) +
                annotate("text",
                        x = nrow(temp_data) / 2,
                        y = bottom_y,
                        label = orders_abundance_temp
                        ) +
                annotate("text",
                        x = nrow(temp_data) / 2,
                        y = upper_y,
                        label = paste0(
                            percent_proteins * 100,
                            " % of proteins represented."
                            )
                        )+
                geom_point(colour = "#FC766AFF", alpha = 0.75, shape = 21)

        } else{
            temp_plot <- ggplot(temp_data, aes(x = vec_temp, y = temp)) +
                ggtitle(colnames(rank_groups)[i]) +
                theme_bw() +
                ylab(expression("log"[10] * "(Intensity)")) +
                xlab("Protein Abundance Rank")+
                geom_point(colour = "#FC766AFF", alpha = 0.75, shape = 21)
            }

        pl[[i]] <- temp_plot

        rm(temp)
        rm(vec_temp)
        rm(limits_row)
        rm(upper_y)
        rm(bottom_y)
        rm(orders_abundance_temp)
    }

    gridExtra::marrangeGrob(
        grobs = pl,
        ncol = columns_grid, nrow = rows_grid, top = NULL
        )
}
BioAlvaro/MQviewer documentation built on Jan. 11, 2022, 8:25 p.m.