figure2a: miR34a asRNA expression in HCT116 and HEK293t upon...

In GranderLab/miR34a_asRNA_project: The miR34AasRNAproject package facilitates the transparency and reproducibility of the miR34a asRNA project and associated publication.

Description

All cell lines were cultured at 5 in DMEM high glucose (Hyclone) and HCT116 cells in McCoy<e2><80><99>s 5a (Life Technologies). All growth mediums were supplemented with 10 heat-inactivated FBS and 50 <ce><bc>g/ml of streptomycin and 50 <ce><bc>g/ml of penicillin. Cells were plated at 300,000 cells per well in a 6-well plate and cultured overnight. The following day cells were treated with 0, 100, 200, or 500 ng/ml doxorubicin for 24hrs. RNA was extracted using the RNeasy mini kit (Qiagen) and subsequently treated with DNase (Ambion Turbo DNA-free, Life Technologies). 500ng RNA was used for cDNA synthesis using MuMLV (Life Technologies) and a 1:1 mix of oligo(dT) and random nanomers. QPCR was carried out using KAPA 2G SYBRGreen (Kapa Biosystems) using the Applied Biosystems 7900HT machine with the cycling conditions: 95 <c2><b0>C for 3 min, 95 <c2><b0>C for 3 s, 60 <c2><b0>C for 30 s.

Format

A tibble with 48 rows and 6 variables:

'Biological Replicate'

Numeric; Indicates the biological replicate of the sample.

'Cell line'

Character; Indicating the cell line the data corresponds to.

Treatment

Character; Indicates the treatment type.

gene

Character; indicates the gene that the Ct values correspond to.

Ct1

Numeric; Ct value corresponding to the first technical replicate.

Ct2

Numeric; Ct value corresponding to the 2nd technical replicate.

...

Examples

getData('Figure 2a')

GranderLab/miR34a_asRNA_project documentation built on May 26, 2019, 7:26 a.m.