supp_figure5c: Monitoring proliferation of miR34a asRNA overexpressings...

In GranderLab/miR34a_asRNA_project: The miR34AasRNAproject package facilitates the transparency and reproducibility of the miR34a asRNA project and associated publication.

Description

20^4 PC3 cells, either miR34a asRNA overexpressing or mock, were harvested and stained in 1 ml PBS with 5uM CellTrace Violet (Invitrogen) for 5min and subsequently seeded in 12 well plates. Time 0 measurements were taken once cells had attatched and treatments with RPMI (Gibco, life technology) 10 FBS, RPMI 0.1 cells. RPMI mediums were all additionally supplemented with 2mM L-glutamine and 50ug/ml Penicillin-Streptomycin and 10 incubated for the indicated times before harvesting and CellTrace Violet was quantified via flow cytometry (Sony SH800S Cell Sorter). Time 0 was performed in biological triplicate and technical duplicate whereas all other time points were performed in biological triplicates with one technical replicate.

Format

A tibble with 976785 rows and 9 variables:

Type

Character; Indicates if the sample is stained or an unstained control.

Time

Character; Indicates the time the sample was harvested.

'Cell line'

Character; indicates the cell line that the values correspond to.

Condition

Character; indicates the culturing conditions for the sample.

'Technical replicate

Numeric; Indicates the technical replicate.

'Biological replicate'

Numeric; Indicates the biological replicate.

'FSC-A'

Numeric; Value for forward scatter area.

'BSC-A'

Numeric; Value for back scatter area

Violet

Numeric; Fluorescence intensity for cellTrace Violet.

...

Examples

getData('Supplementary Figure 5c')

GranderLab/miR34a_asRNA_project documentation built on May 26, 2019, 7:26 a.m.